HPLC法同时测定杏苏止咳糖浆中5种成分  

Simultaneous determination of five consituents in Xingsu Zhike Syrup by HPLC

作　　者：李运 白贺明 邱国玉 石晓峰[2] 戴忠[3] LI Yun;BAI He-ming;QIU Guo-yu;SHI Xiao-feng;DAI Zhong(Lanzhou Municipal Institute for Food and Drug Control/Gansu Provincial Engineering Research Center for Monitoring Exogenous Harmful Residues in Traditional Chinese Medicines,Lanzhou 730000,China;Gansu Provincial Academy of Medical Science,Lanzhou 730050,China;National Institutes for Food and Drug Control,Beijing 100050,China)

机构地区：[1]兰州市食品药品检验检测研究院/甘肃省种植中药材外源性污染物监测工程研究中心,甘肃兰州730000 [2]甘肃省医学科学研究院,甘肃兰州730050 [3]中国食品药品检定研究院,北京100050

出　　处：《中成药》2021年第8期2002-2005,共4页Chinese Traditional Patent Medicine

基　　金：国家药品标准物质协作研究与标定(2020⁃W0204001);兰州市2019年第一批科技计划项目(2019⁃1⁃60)。

摘　　要：目的建立HPLC法同时测定杏苏止咳糖浆(苦杏仁、紫苏叶、前胡等)中5种成分的含量。方法该药物80%甲醇提取液的分析采用CAPCELL PAK C_(18) MG色谱柱(250 mm×4.6 mm,5μm);流动相水(含0.05%磷酸)⁃乙腈,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长210、237、321 nm。结果苦杏仁苷、甘草苷、甘草酸、白花前胡甲素、白花前胡乙素在各自范围内线性关系良好(r>0.9900),平均加样回收率95.3%~101.2%,RSD 0.66%~2.57%。结论该方法稳定、准确、可靠,可用于杏苏止咳糖浆的质量控制。AIM To establish an HPLC method for the simultaneous content determination of five constituents in Xingsu Zhike Syrup(Armeniacae Semen Amarum,Perillae Folium,Peucedani Radix,etc.).METHODS The analysis of 80%methanol extract of this drug was performed on a 30℃thermostatic CAPCELL PAK C_(18) MG column(250 mm×4.6 mm,5μm),with the mobile phase comprising of water(containing 0.05%phosphoric acid)⁃acetonitrile flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 210,237,321 nm.RESULTS Amygdalin,liquiritin,glycyrrhizic acid,praeruptorin A and praeruptorin B showed good linear relationships within their own ranges(r>0.9900),whose average recoveries were 95.3%-101.2%with the RSDs of 0.66%-2.57%.CONCLUSION This stable,accurate and reliable method can be used for the quality control of Xingsu Zhike Syrup.

关 键 词：杏苏止咳糖浆 苦杏仁苷 甘草苷 甘草酸 白花前胡甲素 白花前胡乙素 HPLC 

分 类 号：R927.2􀆰[医药卫生—药学]