复方牦牛骨粉对骨质疏松大鼠的影响  

Effects of compound yak bone powder on osteoporosis in rats

作　　者：郜靓 梁丹妮 杨红霞[1,2] 肖远灿[1,2] 魏立新[1,2] 杜玉枝[1,2] GAO Liang;LIANG Dan-ni;YANG Hong-xia;XIAO Yuan-can;WEI Li-xin;DU Yu-zhi(Qinghai Key Laboratory for Pharmacology and Safety Evaluation of Tibetan Medicine,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China;Key Laboratory for Research of Tibetan Medicine,Chinese Academy of Sciences,Xining 810008,China;University of Chinese Academic of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院西北高原生物研究所,青海省藏药药理学与安全性评价研究重点实验室,青海西宁810008 [2]中国科学院藏药重点实验室,青海西宁810008 [3]中国科学院大学,北京100049

出　　处：《中成药》2021年第8期2035-2040,共6页Chinese Traditional Patent Medicine

基　　金：青海省基础研究计划项目(2019⁃2J⁃7026)。

摘　　要：目的探讨复方牦牛骨粉对维甲酸诱导的高转换型骨质疏松症大鼠的影响。方法SD大鼠灌胃维甲酸(70 mg/kg)14 d(每天1次)建立骨质疏松模型后,灌胃给予不同剂量复方牦牛骨粉(每天1次),设立阳性对照组、模型组、空白组和复方牦牛骨粉低、中、高剂量组,连续给药4周。取大鼠血清、腰椎及股骨,进行骨转化标志物、骨密度和骨组织形态学检测。结果与模型组比较,给药组大鼠体质量增长率增加,股骨及脊椎骨密度升高(P<0.05,P<0.01);复方牦牛骨粉中、高剂量组血清Ca2+、ALP水平及股骨、腰椎BS/TV降低(P<0.05,P<0.01),股骨Tb.Th升高(P<0.01),中剂量组Tb.Sp降低(P<0.05),中、高剂量组腰椎BV/TV及低剂量组Tb.N升高(P<0.05)。结论复方牦牛骨粉可通过增加骨形成、改善骨代谢来防治维甲酸诱导的高转换型骨质疏松症。AIM To explore the effects of compound yak bone powder on rats with osteoporosis induced by retinoic acid.METHODS We had a positive control group,a model group and groups for interventions of low⁃dose,moderate⁃dose,and high⁃dose compound yak bone powder administration.After induction into the osteoporosis models with 14 days(once daily)retinoic acid treatment(70 mg/kg),SD rats were intragastrically given different doses of compound yak bone powder once daily for 4 consecutive weeks.After the experiment,the rats had their serum,lumbar spine and femur procured for identification of bone transformation marker,bone density(BMD)and bone histomorphology.RESULTS Compared with the model group,the groups intervened with the drugs shared increased body weight growth rate;increased bone density in the femur and spine(P<0.05,P<0.01).Groups intervened with moderate⁃dose or high⁃dose compound yak bone powder demonstrated increased serum levels of Ca2+and ALP(P<0.05,P<0.01);down⁃regulated BS/TV of femoral and lumbar spine(P<0.05,P<0.01);and elevated Tb.Th(P<0.01)and BV/TV(P<0.05).Additionally,markedly lower Tb.S level in the moderate⁃dose group(P<0.05)and higher Tb.N level in the low⁃dose group were both observed(P<0.05).CONCLUSION Compound yak bone powder highlights the treatment of retinoic acid⁃induced osteoporosis.

关 键 词：复方牦牛骨粉 骨质疏松症 骨密度 骨转化标志物 骨组织形态 

分 类 号：R285.5􀆰[医药卫生—中药学]