芥子咳喘贴对支气管炎模型小鼠药效学作用观察  

Observation of Pharmacological Actions of Jiezi Kechuan Plaster on Model Mice with Bronchitis

作　　者：王道涵[1] 乔艳雪 袁晓雯[2] 赵荣华[3] 王素梅[1] 马雅銮[2] WANG Daohan;QIAO Yanxue;YUAN Xiaowen;ZHAO Ronghua;WANG Sumei;Ma Yaluan(Department of Pediatrics,Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China;Institute of Basic Theory for Chinese Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China;Institute of Chinese Meteria Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]北京中医药大学东方医院儿科,北京100078 [2]中国中医科学院中医基础理论研究所,北京100700 [3]中国中医科学院中药研究所,北京100700

出　　处：《中药新药与临床药理》2021年第7期920-924,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：北京市科委“十病十药”研发项目(Z141100002214009)。

摘　　要：目的观察芥子咳喘贴对支气管炎模型小鼠的镇咳、祛痰、抗炎的药效学作用。方法将80只C57BL/6小鼠随机分为空白对照组、平喘止咳贴组、芥子咳喘贴常规剂量组、芥子咳喘贴高剂量组,每组20只。采用氨水引咳法制备小鼠咳嗽模型、呼吸道酚红排泌实验观察排痰情况、脂多糖(LPS)鼻饲诱导法观察小鼠支气管炎性反应。药物干预后,测定5 min内模型小鼠咳嗽次数和潜伏时间、分光光度计检测肺泡灌洗液中酚红浓度、微量样本多指标流式蛋白定量技术(CBA法)检测小鼠外周血与灌洗液中白细胞介素(IL)-6、IL-10、IL-12、干扰素γ(IFN-γ)、肿瘤坏死因子α(TNF-α)、单核细胞趋化蛋白1(MCP-1)水平。结果与空白对照组比较,芥子咳喘贴常规剂量组、高剂量组及平喘止咳贴组咳嗽次数明显减少,差异有统计学意义(P<0.05);与平喘止咳贴组比较,芥子咳喘贴常规剂量组、高剂量组咳嗽次数明显减少,差异有统计学意义(P<0.05);而常规剂量组、高剂量组之间差异无统计学意义(P>0.05)。与空白对照组比较,芥子咳喘贴常规剂量组、高剂量组明显延长咳嗽潜伏时间,差异有统计学意义(P<0.05),但二者之间比较无明显差异(P>0.05)。与空白对照组比较,芥子咳喘贴常规剂量组、高剂量组肺泡灌洗液酚红浓度明显增加,差异有统计学意义(P<0.05);高剂量组肺泡灌洗液酚红浓度明显高于常规剂量组,差异有统计学意义(P<0.05)。与空白对照组比较,常规剂量组血浆炎性因子MCP-1、IL-6水平明显下降,差异有统计学意义(P<0.05);肺泡灌洗液中炎症因子水平比较,各组差异无统计学意义(P>0.05)。结论芥子咳喘贴具有良好的镇咳、祛痰作用,可降低外周血炎症因子MCP-1、IL-6水平,芥子咳喘贴常规剂量组、高剂量组药效学作用总体相当。Objective To observe antitussive,expectorant and anti-inflammatory effects of Jiezi Kechuan plaster on model mice with bronchitis.Methods 80 healthy C57BL/6 mice were randomly divided into four groups:blank control(BC)group,Pingchuan Zhike plaster(PZP)group,Jiezi Kechuan plaster conventional dose(c-JKP)group and Jiezi Kechuan plaster high dose(h-JKP)group(n=20 in each group).Cough model mice induced by ammonia liquor were established,the expectorant model mice induced by phenol red and the inflammatory model mice induced by nasal intake of lipopolysaccharidenasal(LPS)were used.After drug intervention,cough frequency and latency were measured in 5 minutes,the concentration of phenol red in bronchoalveolar lavage fluid(BALF)was measured by spectrophotometer.The expressions of IL-6,IL-10,IL-12,IFN-γ,TNF-αand MCP-1 in peripheral blood and BALF were detected by cytometric bead array(CBA).Results Compared with BC group,the frequency of cough decreased significantly in PZP group,c-JKP group and h-JKP group(P<0.05).Compared with PZP group,the frequency of cough decreased significantly in c-JKP group and h-JKP group(P<0.05),whereas it showed no significant difference between JKP groups(P>0.05).Compared with BC group,cough latency was significantly prolonged in c-JKP group and h-JKP group(P<0.05),with no significant difference between them(P>0.05);Compared with BC group,the concentrations of phenol red were significantly increased in c-JKP group and h-JKP group(P<0.05),and the latter concentration was much higher than that of the former(P<0.05).Compared with BC group,the plasma levels of MCP-1,IL-6 in c-JKP group decreased much significantly(P<0.05),but inflammatory factors in BALF showed no significant differences between groups(P>0.05).Conclusion Jiezi Kechuan plaster exerted significant pharmacological effects on antitussive and expectorant actions,decreased the levels of MCP-1,IL-6 in peripheral blood.On the whole,the effects between c-JKP group and h-JKP group are similar.

关 键 词：芥子咳喘贴 镇咳 祛痰 抗炎 支气管炎 小鼠 

分 类 号：R285.5[医药卫生—中药学]