红花蜂花粉多糖APBPC-3对前列腺癌DU145细胞增殖的影响  

Effect of bee pollen of carthamus tinctorius polysaccharide APBPC-3 on the proliferation of DU145 cells in prostate cancer

作　　者：李淑芳 史天洁 左绍远 LI Shu-fang;SHI Tian-jie;ZUO Shao-yuan(College of Basic Medicine,Dali University,Dali,Yunnan 671000,China)

机构地区：[1]大理大学基础医学院,云南大理671000 [2]云南省昆虫生物医药研发重点实验室

出　　处：《实用医药杂志》2021年第7期621-625,F0003,共6页Practical Journal of Medicine & Pharmacy

基　　金：国家自然科学基金资助项目(31460231);云南省昆虫生物医药研发重点实验室项目(2014-10)。

摘　　要：目的探讨碱提红花蜂花粉多糖组分(APBPC-3)对前列腺癌DU145细胞增殖的影响及其作用机制。方法以不同浓度APBPC-3处理DU145细胞,采用CCK-8法检测细胞增殖情况,并观察DU145细胞形态变化;采用RT-PCR、Western blot法检测PI3K/Akt信号通路中PI3K、Akt-1、PTEN、P53、Caspase-3、Bcl-2及Bax的表达。结果 APBPC-3具有剂量-时间依赖性的抗增殖作用,可使DU145细胞发生明显形态学改变,对DU145前列腺癌细胞的48 h IC50值为304.039μg/ml。RT-PCR和Western blot结果表明,APBPC-3可下调PI3K/Akt信号通路PI3K、Akt-1、Bcl-2的表达,上调PTEN、P53、Caspase-3和Bax的表达。结论 APBPC-3可抑制前列腺癌DU145细胞的增殖,其作用机制可能是通过调控PI3K/Akt信号通路实现的。Objective To investigate the effect of bee pollen of carthamus tinctorius polysaccharide APBPC-3 on the proliferation of DU145 cells in prostate cancer and its mechanism.Methods The DU145 cells were treated with APBPC-3 at different concentrations.The proliferation of DU145 cells was detected by CCK-8 method,and the morphological changes were observed;The expressions of PI3K,AkT-1,PTEN,P53,Caspase-3,Bcl2 and Bax in the PI3K/Akt signaling pathway were detected by RT-PCR and Western blot.Results APBPC-3 had a dose-time dependent antiproliferative effect and resulted in significant morphological changes in DU145 cells,with a 48h IC50 value of 304.039g/ml against DU145 prostate cancer cells.The results of RT-PCR and Western blot showed that APBPC-3 could down-regulate the expression of PI3K/Akt signaling pathway related proteins PI3K,Akt-1 and Bcl-2,as well as up-regulate the expression of PTEN,P53,Caspase-3 and Bax.Conclusion APBPC-3 can inhibit the proliferation of prostate cancer DU145 cells,and its mechanism may be realized by regulating the PI3K/Akt signaling pathway.

关 键 词：碱提 红花蜂花粉多糖 DU145细胞 PI3K/Akt信号通路 

分 类 号：R285.5[医药卫生—中药学]