海南黎族人群VEGF基因多态性和嚼槟榔习惯在2型糖尿病中的作用  

Role of Polymorphisms of VEGF and Areca Nut Chewing in Type 2 Diabetes Mellitus in Li Population of Hainan Province.

作　　者：陈宗存[1] 符沙沙[2] 陈金逸 张鸾 陈清华[1] 吴祖荣 Chen Zongcun;Fu Shasha;Chen Jinyi(Department of Endocrinology,The Second Affiliated Hospital of Hainan Medical University,Hainan 570311,China)

机构地区：[1]海南医学院附属第二医院内分泌科,海口570311 [2]海口市人民医院呼吸与危重症医学科,570208 [3]海口市第三人民医院内分泌科,570100

出　　处：《医学研究杂志》2021年第8期100-102,117,共4页Journal of Medical Research

基　　金：海南省卫生计生行业科研项目(16A200071,20A200489)。

摘　　要：目的探讨海南黎族人群血管内皮生长因子(VEGF)-460C/T多态性和嚼槟榔习惯与2型糖尿病(T2DM)的联系及交互作用。方法选取2016年1月-2020年1月在海南地区住院的116例黎族2型糖尿病患者作为T2DM组,180例黎族健康体检者作为对照组。收集研究对象的年龄、性别、吸烟、嚼槟榔习惯、血压、血糖、血脂等资料,抽血采用多聚酶链反应-限制性片段长度多态性(PCR-RFLP)法检测VEGF-460C/T基因型分布情况。采用Logistic回归分析各基因型与2型糖尿病易感性的关系及和嚼槟榔习惯的交互作用。结果T2DM组的C等位基因比例高于对照组(64.7%vs 53.9%,P<0.05)。Logistic回归分析显示,C基因携带者(CC型/CT型)患病风险是非C基因携带者(TT型)的1.89倍(OR=1.89,95%CI:1.01-3.57)。VEGF基因多态性和嚼槟榔习惯具有交互作用(RERI=30.24,95%CI:19.20-99.68),CC/CT型且有嚼槟榔习惯者患T2DM的风险是TT基因型且无嚼槟榔习惯者的2.95倍(OR=2.95,95%CI:1.50-5.81)。结论VEGF-460C/T多态性和海南黎族人群患2型糖尿病有关,同时有CC/CT基因型和嚼槟榔习惯者患T2DM的风险更高。Objective To investigate the relationship between 460C/T site polymorphisms of vascular endothelial growth factor(VEGF)and type 2 diabetes mellitus(T2DM)and the interaction with areca nut chewing in Hainan Li population.Methods Totally 116 cases of patients hospitalized and diagnosed with T2DM from January 2016 to January 2020 were enrolled as the T2DM group and 180 volunteers who had physical examination in the same period were enrolled as the control group.Data of age,sex,smoking,areca nut chewing,blood pressure,glycosylated hemoglobin(HbA1c)and blood lipid were collected.Genomic DNA was extracted from peripheral blood,and the polymorphisms of VEGF gene 460C/T site were examined by polymerase chain reaction based restriction fragment length polymorphism(PCR-RFLP).Results In the T2DM group,the frequency of C allele at 460C/T of VEGF gene were significantly higher than those of the control group(64.7%vs 53.9%,P<0.05).Logistic regression analysis showed that there was increased risk of developing T2DM for CC/CT genotype carriers compared with TT(Adjusted OR=1.89,95%CI:1.01-3.57).The OR of the additive interaction indexes RERI between VEGF polymorphisms and areca nut chewing on T2DM development was 30.24(95%CI:19.20-99.68).Conclusion VEGF-460C/T polymorphism is associated with T2DM in Li population of Hainan,and those with CC/CT genotype and areca chewing have a higher risk of developing T2DM.

关 键 词：血管内皮生长因子 基因多态性 2型糖尿病 槟榔 

分 类 号：R587.1[医药卫生—内分泌]