青霉素对银屑病模型小鼠肠道菌群变化及免疫功能调节作用的实验研究  

Experimental study of penicillin on intestinal flora changes and immune function regulation in model mice with psoriasis

作　　者：金曌 王飞[2] 苏慧[1] 张峻 殷翘 JIN Zhao;WANG Fei;SU Hui;ZHANG Jun;YIN Qiao(Dermatology Department,Wuhan No.1 Hospital,Wuhan 430022,China)

机构地区：[1]武汉市第一医院皮肤科,武汉430022 [2]武汉市第一医院针灸科,武汉430022

出　　处：《中国免疫学杂志》2021年第15期1814-1819,共6页Chinese Journal of Immunology

基　　金：湖北省卫生健康委科研项目(WJ2019M025)。

摘　　要：目的:探究青霉素对银屑病模型小鼠肠道菌群变化及免疫功能调节作用的影响。方法:24只4~6周龄雌性BALB/c小鼠随机分为青霉素组(备皮后外涂5%咪喹莫特软膏诱导银屑病模型,腹腔注射青霉素)、模型组(备皮后外涂5%咪喹莫特软膏诱导银屑病模型,腹腔注射生理盐水)和对照组(备皮后外涂凡士林软膏,腹腔注射生理盐水),每组8只,皮损面积和严重程度指数(PASI)评分和HE染色评估各组小鼠皮损情况,流式细胞术检测小鼠血清免疫细胞因子CD4^(+)T细胞、CD8^(+)T细胞、CD11c^(+)树突状细胞(DCs)水平,ELISA检测血清炎症细胞因子IL-17A、IL-17F、IL-23、IL-22水平,并分析肠道菌群变化。结果:对照组小鼠背部皮肤无任何明显变化,模型组小鼠第2天起,背部皮肤出现红斑、鳞片和浸润,并逐渐明显,第7天,皮肤表现与人斑块型银屑病相似,病理可见皮肤明显角化、棘突及炎症细胞浸润,青霉素组小鼠背部皮肤仅可见少量红斑和薄鳞片,无明显浸润,皮肤病理变化明显减轻;模型组第2、4、7天PASI评分明显高于对照组(P<0.05),青霉素组第2、4、7天PASI评分明显低于模型组(P<0.05);模型组CD4^(+)T细胞、CD8^(+)T细胞百分比明显低于对照组(P<0.05),CD11c^(+)DCs百分比明显高于对照组(P<0.05),青霉素组CD4^(+)T细胞百分比明显高于模型组(P<0.05),CD11c^(+)DCs百分比明显低于模型组(P<0.05),CD8^(+)T细胞百分比与模型组差异无统计学意义(P>0.05);模型组IL-17A、IL-17F、IL-22、IL-23水平明显高于对照组(P<0.05),青霉素组IL-17A、IL-17F、IL-22、IL-23水平明显低于模型组(P<0.05);模型组乳酸杆菌、双歧杆菌、肠球菌及肠杆菌数与对照组差异无统计学意义(P>0.05),青霉素组乳酸杆菌、双歧杆菌数明显少于模型组(P<0.05),肠球菌、肠杆菌数与对照组差异无统计学意义(P>0.05),青霉素组OTU、Chao1、ACE、Shannon值明显低于模型组(P<0.05),Simpson值明显高于模型�Objective:To investigate effects of penicillin on intestinal flora changes and immune function regulation in model mice with psoriasis.Methods:24 female BALB/c mice aged 4~6 weeks were randomly divided into penicillin group(after skin preparation,external application of 5%imiquimod ointment for inducing psoriasis model,and intraperitoneal injection of penicillin),model group(after skin preparation,external application of 5%imiquimod ointment for inducing psoriasis model,and intraperitoneal injection of saline)and control group(after skin preparation,external application of vaseline ointment,and intraperitoneal injection of saline),8 cases in each group.Percutaneous area and severity index(PASI)score and HE staining to evaluate skin lesions of mice in each group.Flow cytometry to detect levels of serum immune cytokines CD4^(+)T cells,CD8^(+)T cells,CD11c+dendritic cells(DCs),and ELISA to detect serum inflammatory cytokines IL-17A,IL-17F,IL-23,IL-22 levels,and changes of intestinal flora were analyzed.Results:There was no obvious change in back skin of mice in control group.From 2nd d,erythema,scales and infiltration appeared on back skin and gradually became obvious in model group,and at 7 d,skin appearance was similar to human plaque psoriasis,and pathology showed obvious skin keratinization,spinous processes and inflammatory cell infiltration.Back skin of mice in penicillin group showed only a small amount of erythema and thin scales without obvious infiltration,and pathology showed that skin changes were significantly reduced.PASI scores in model group at 2,4,and 7 d were significantly higher than those in control group(P<0.05),and PASI scores in penicillin group at 2,4,and 7 d were significantly lower than those in model group(P<0.05).Percentages of CD4^(+)T cells and CD8^(+)T cells in model group were significantly lower than those in control group(P<0.05),while percentage of CD11c+DCs was significantly higher than that in control group(P<0.05),and percentage of CD4^(+)T cells in penicillin group was signific

关 键 词：青霉素 银屑病 免疫功能 肠道菌群 炎症因子 

分 类 号：R310.51[医药卫生—基础医学]