银屑病患者血清对真皮间充质干细胞基因表达及功能的影响  

Effect of serum in patients with psoriasis on gene expression and function of dermal mesenchymal stem cells

作　　者：王方迪 侯瑞霞[2] 赵新程[2] 李俊琴[2] 张开明[2] 李新华(指导)[2] WANG Fang-Di;HOU Rui-Xia;ZHAO Xin-Cheng;LI Jun-Qin;ZHANG Kai-Ming;LI Xin-Hua(Second Hospital of Shanxi Medical University,Taiyuan 030009,China)

机构地区：[1]山西医科大学第二临床医学院,太原030009 [2]山西医科大学附属太原中心医院皮肤科,免疫性皮肤病干细胞山西省重点实验室,太原030009

出　　处：《中国免疫学杂志》2021年第15期1867-1871,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(81602768);山西省自然科学基金(201801D221441)资助。

摘　　要：目的:探讨银屑病患者血清对真皮间充质干细胞(DMSCs)免疫功能的影响。方法:用银屑病患者血清作用于正常DMSCs,采用实时荧光定量聚合酶链反应(real-time-PCR)检测IL-10、PGE_(2)、TLR4的mRNA表达水平,同时检测作用后的DMSCs对PHA活化的T细胞增殖的抑制能力。结果:银屑病患者血清作用后的DMSCs表达IL-10、PGE_(2)、TLR4的mRNA水平较正常对照组降低,且对PHA活化的T细胞增殖的抑制作用减弱,差异具有统计学意义(P<0.05)。结论:银屑病患者血清作用于DMSCs后,其血清中升高的炎症因子会导致DMSCs表达的TLR4、PGE_(2)、IL-10减少,进而降低间充质干细胞的免疫调节功能。Objective:To investigate the effect of serum on the immune function of dermal mesenchymal stem cells(DMSCs)in patients with psoriasis.Methods:The normal dermal mesenchymal stem cells were treated with psoriatic serum,expression levels of IL-10,PGE_(2),TLR4 mRNA were detected by real-time-PCR,at the same time,the inhibition of DMSCs on the proliferation of PHA activated T cells was detected.Results:The mRNA expression levels of IL-10,PGE_(2) and TLR4 in the dermal mesenchymal stem cells treated with serum in psoriasis were lower than that in the normal controls,the inhibition of DMSCs on the proliferation of PHA activated T cells was weakened(P<0.05).Conclusion:After the serum of patients with psoriasis acts on the dermal mesenchymal stem cells,the increased inflammatory factors in their serum will lead to the decrease of TLR4,PGE_(2) and IL-10 expression of mesenchymal stem cells and then reduce the immunoregulatory function of mesenchymal stem cells.

关 键 词：银屑病 间充质干细胞 细胞因子 免疫调节 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]