不同炮制方法对雷公藤中雷公藤内酯甲和雷公藤甲素的影响  

Study on the Influence of Different Processed Methods on the Wilforlide A and Triptolide in Tripterygium wilfordii

作　　者：张奉苏 房克慧 陈宏降[2] 田静 张柳 罗益远 ZHANG Feng-su;FANG Ke-hui;CHEN Hong-jiang;TIAN Jing;ZHANG Liu;LUO Yi-yuan(Yangzhou Institute for Drug Control,Yangzhou 225000,China;College of Traditional Chinese Medicine,Zhejiang Pharmaceutical College,Ningbo,315500,China;Nanjing University of Chinese Medicine,Nanjing 210023 China)

机构地区：[1]扬州市食品药品检验检测中心,扬州225000 [2]浙江医药高等专科学校中药学院,宁波315500 [3]南京中医药大学,南京210023

出　　处：《人参研究》2021年第4期18-21,共4页Ginseng Research

基　　金：宁波市公益类科技计划,项目编号:2019C50064;浙江省中医药优秀青年人才项目,项目编号:2020ZQ049;浙江医药高等专科学校校课题,项目编号:2019013;ZJCSR2020004;浙江中医药大学省重点建设高校优势特色学校(中药学)开放基金资助,项目编号:ZYAOX2018028;浙江省大学生新苗人才计划,项目编号:2020R424003;2021R460004。

摘　　要：目的建立同时测定雷公藤中雷公藤内酯甲和雷公藤甲素含量的分析方法,比较不同炮制方法对雷公藤药材中雷公藤内酯甲和雷公藤甲素含量的影响及炮制前后的变化。方法采用外标法,以0.1%磷酸乙腈(A)-0.2%三乙胺水溶液(B)为流动相,梯度洗脱;检测波长为210 nm;流速为1.0 mL/min。结果雷公藤内酯甲和雷公藤甲素分别在7.13~285.20 μg/mL(r=0.9990)和6.13~245.20μg/mL(r=0.9999)范围内呈良好的线性关系,加样回收率为96.24%~104.53%,RSD为0.9%~2.7%。不同炮制方法的雷公藤药材中雷公藤内酯甲和雷公藤甲素的含量差异显著,其中酒炙方法对上述两种成分影响较大。结论本实验揭示炮制方法对雷公藤的雷公藤甲素和雷公藤内酯甲的含量有一定的影响,为后续雷公藤减毒增效研究提供基础资料。Objective To establish a high performance liquid chromatographic method for the determination of wilforlide A and triptolide in Tripterygium wilfordii,and investigate the influence of wilforlide A and triptolide of the different processing Methods of Tripterygium wilfordii.Methods Using external standard method,the mobile phase was acetonitrile(0.1%phosphoric acid,A)-triethylamine(0.2%,B),the detection wavelength was 210 nm,and the flow rate was 1.0 mL/min.Results Wilforlide A and TRiptolide had a good linearity in the range of 7.13~285.20μg/mL(r=0.9990)and 6.13~245.20μg/mL(r=0.9999)respectively.The average recoveries of standard addition for the compounds were between 96.24%and 104.53%The different processing methods of Tripterygium wilfordii.had certain influence to 2 kinds of components.The wine processing method has a great influence on the above two components.Conclusion This experiment revealed that the processing method had a certain influence on the main components of Tripterygium wilfordii.,to provide the basic data for the follow-up study on the toxicity and synergism of Tripterygium wilfordii.

关 键 词：雷公藤 高效液相色谱法 炮制方法 雷公藤甲素 雷公藤内酯甲 

分 类 号：R283[医药卫生—中药学]