基于四臂嗅觉仪内轨迹跟踪的昆虫嗅觉行为分析体系  

Insect olfactory behavior assay system based on trajectory tracking in four-quadrant olfactometer

作　　者：孙琳琳 张璇 石佳敏 常学飞 叶恭银[1] 汪芳 SUN Lin-Lin;ZHANG Xuan;SHI Jia-Min;CHANG Xue-Fei;YE Gong-Yin;WANG Fang(State Key Laboratory of Rice Biology,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]浙江大学农业与生物技术学院,水稻生物学国家重点实验室,杭州310058

出　　处：《昆虫学报》2021年第7期828-839,共12页Acta Entomologica Sinica

基　　金：浙江大学实验技术项目(SJ201810)。

摘　　要：【目的】结合四臂嗅觉仪与轨迹跟踪分析系统,通过分析不同试验条件下黑腹果蝇Drosophila melanogaster的嗅觉行为,建立适合于果蝇等小型昆虫的嗅觉行为实验技术体系。【方法】采用Ethovision XT软件追踪黑腹果蝇在四臂嗅觉仪内的活动轨迹,分析各区域内的累计停留时长、运动速度;同时,考察气流速度和味源设置对苹果醋引诱及蓝桉Eucalyptus globulus叶油驱避黑腹果蝇效果的影响。【结果】适合于黑腹果蝇嗅觉行为分析的气流速度为300~1200 mL/min;300 mL/min时黑腹果蝇个体间运动速度最为均一,因此推荐最适气流速度大小为300 mL/min。味源设置不会改变黑腹果蝇对味源物的嗅觉行为反应,但对味源物的引诱指数有一定的影响,单一臂放置味源物试验效果更佳。单头测试的实验效果优于多头试验。【结论】本研究建立的基于轨迹跟踪的嗅觉行为分析体系可用于昆虫信息化合物有效成分的筛选鉴定。在合适的气流条件下,单一臂设置味源化合物逐头进行测试试验效果更佳;多头试验可用于化合物的初步筛选。【Aim】The study aims to establish a suitable olfactory behavior assay system for small insects such as Drosophila melanogaster based on trajectory tracking in four-quadrant olfactometer by analyzing the olfactory behavior of D.melanogaster under different experimental conditions.【Methods】The movement trajectory of D.melanogaster in the four-quadrant olfactometer was tracked,and the residence time and moving speed within each field arena was analyzed using Ethovision XT software.Meanwhile,the effects of airflow velocity and ordor placement on the attraction of apple vinegar and the repellence of Eucalyptus globulus leaf oil to D.melanogaster were investigated.【Results】The airflow velocity suitable for olfactory behavior analysis of D.melanogaster was from 300 to 1200 mL/min,and 300 mL/min airflow was recommended as the optimal airflow velocity at which the moving speed of D.melanogaster individuals was more uniform.Ordor placement had no effect on the olfactory behavior of D.melanogaster,but had some impact on the attraction index of ordor.Single ordor showed better efficiency.Meanwhile,the efficiency of single individual test was better than that of multiple individual test.【Conclusion】The olfactory behavior assay system based on trajectory tracking developed in this study can be used for screening and identifying effective components in semiochemical compounds of insects.Better efficiency can be obtained by testing each insect individually with a suitable airflow velocity and single ordor.Multiple individual test could be used in the primary screening of compounds.

关 键 词：黑腹果蝇 嗅觉行为 气流速度 味源设置 停留时长 引诱指数 轨迹跟踪 四臂嗅觉仪 

分 类 号：Q968[生物学—昆虫学]