5′-硝基水杨基荧光酮分光光度法测定维生素B_(12)中钴的含量  

Spectrophotometric Determination of Cobalt in Vitamin B_(12) with 5′-Nitro-Salicylfluorone

作　　者：汤家华 程乐华[1,2] TANG Jia-hua;CHENG Le-hua(School of Chemistry and Material Engineering,Chaohu University,Chaohu Anhui 238024;Institute of Coordination Chemistry,Chaohu University,Chaohu Anhui 238024)

机构地区：[1]巢湖学院化学与材料工程学院,安徽巢湖238024 [2]巢湖学院配位化学研究所,安徽巢湖238024

出　　处：《巢湖学院学报》2021年第3期78-85,共8页Journal of Chaohu University

摘　　要：以5′-硝基水杨基荧光酮(5′-NSAF)为显色剂,建立了分光光度法测定钴的新方法。研究表明:在pH为9.2的NH_(3)·H_(2)O-NH_(4)Cl缓冲介质中,在表面活性剂TritonX-100作用下,钴(Ⅱ)与5′-硝基水杨基荧光酮能生成一种红色配合物,配合物在546 nm处有最大吸收,表观摩尔吸光系数为3.7×10^(4)L·mol^(-1)·cm^(-1),检出限(3s/k)为5.64×10^(-3)mg/L,钴(Ⅱ)的质量浓度在0~0.5μg/mL范围内与吸光度A有良好的线性关系。方法应用于维生素B_(12)注射液中钴含量的测定,结果满意。A new method for the determination of cobalt was established by spectrophotometry with 5′-nitro-salicylfluorone(5′-N-SAF)as coloring agent.In the buffer solution of NH_(3)·H_(2)O-NH4Cl at pH 9.2,cobalt(Ⅱ)reacted with 5′-N-SAF and formed a red complex in the presence of TritonX-100.The apparent molar absorptivity was 3.7×10^(4) L·mol^(-1)·cm^(-1) at 546nm;the detection limit(3s/k)was 5.64×10^(-3)mg/L;Beer′s law was obeyed for the mass concentration of cobalt(Ⅱ)in range of 0~0.5μg/mL.The method was applied to the determination of cobalt content in Vitamin B12 injections,and the results were satisfactory.

关 键 词：5′-硝基水杨基荧光酮 分光光度法 维生素B_(12) 钴 

分 类 号：O657.3[理学—分析化学]