松辽黑猪OAS 1基因多态性及其与繁殖性状的关联分析  

Polymorphism of OAS 1 Gene and Its Association with Reproductive Traits in Songliao Black Pigs

作　　者：张云鹏 张琪[2] 刘庆雨[2] 张志彬[2] 张庆[2] 张净博 张树敏 ZHANG Yunpeng;ZHANG Qi;LIU Qingyu;ZHANG Zhibin;ZHANG Qing;ZHANG Jingbo;ZHANG Shumin(College of Animal Science and Technology,Jilin Agricultural University,Changchun 130124,China;Jilin Academy of Agricultural Sciences,Changchun 130124,China;Gongzhuling National Agricultural Science and Technology Park Feimas Animal Husbandry Co.,Ltd.,Gongzhuling 136100,China)

机构地区：[1]吉林农业大学动物科学技术学院,长春130124 [2]吉林省农业科学院,长春130124 [3]公主岭国家农业科技园飞马斯牧业有限公司,公主岭136100

出　　处：《中国畜牧兽医》2021年第8期2910-2919,共10页China Animal Husbandry & Veterinary Medicine

基　　金：吉林省科技厅科技攻关项目“生猪高效繁育与现代化养殖技术开发”(20190301036NY);吉林省科技发展计划项目(20191001039XH);吉林省科技厅地方科技创新与扶贫项目(20191001039XH);吉林省优质特色黑猪产业发展现状调研。

摘　　要：为探究寡腺苷酸合成酶1(oligoadenylate synthase 1,OAS1)基因多态性与松辽黑猪繁殖性状的关联性,试验选取130头松辽黑猪母猪为研究对象,利用Sanger直接测序法测序查找OAS 1基因外显子1~8的SNP位点,使用SPSS 19.0软件分析OAS 1基因SNP位点与松辽黑猪繁殖性状的关联性。结果显示,在松辽黑猪OAS 1基因外显子2、3和6上共检测到33个突变位点;其中在外显子2的110 bp处存在1个SNP位点(G110C),存在3种基因型:GG、GC和CC;在外显子3的176 bp处存在1个SNP位点(C176T),存在3种基因型:CC、CT和TT;在外显子6的145 bp处存在1个SNP位点(C145T),存在3种基因型:CC、CA和AA;在166 bp处存在1个SNP位点(G166A),存在3种基因型:GG、GA和AA;在206 bp处存在1个SNP位点(A206G),存在3种基因型:AA、AG和GG。卡方适合性检验结果显示,松辽黑猪OAS 1基因G110C突变位点符合Hardy-Weinberg平衡状态,C176T、C145A、G166A和G206A位点均偏离Hardy-Weinberg平衡状态。群体遗传参数分析结果显示,各SNPs位点遗传杂合度均位于中等水平,为中度多态(0.25<PIC<0.5)。关联分析结果发现,G110C位点GC基因型个体总产仔数、产活仔数和断奶仔猪数均显著高于GG基因型个体(P<0.05);C176T位点CT基因型个体断奶仔猪数显著高于CC基因型个体(P<0.05);C145T位点CC基因型个体总产仔数和产活仔数均显著高于AA基因型个体(P<0.05);G166A位点GA基因型个体断奶仔猪数显著高于GG基因型个体(P<0.05);A206G位点GG基因型个体总产仔数和产活仔数显著高于AA基因型个体(P<0.05)。结果表明,OAS 1基因外显子区存在突变位点,对松辽黑猪部分繁殖性状有显著性影响。This study was aimed to investigate the association of the polymorphism of oligoadenylate synthase 1(OAS1)gene with reproductive traits in Songliao Black pigs.130 Songliao Black pigs were selected as the research objects,the SNPs of OAS 1 gene exons 1-8 were found by Sanger direct sequencing,and the correlation between SNP of OAS 1 gene and reproductive traits of Songliao Black pigs was analyzed using SPSS 19.0 software.The results showed that 33 mutations were detected in exons 2,3 and 6 of OAS 1 gene in Songliao Black pigs.There was a SNP at 110 bp of OAS 1 gene exon 2(G110C),which contained three genotypes of GG,GC and CC.There was a SNP at 176 bp of OAS 1 gene exon 3(G176T),which contained three genotypes of CC,CT and TT.There was a SNP at 145 bp of OAS 1 gene exon 6(C145T),which contained three genotypes of CC,CA and AA.There was a SNP at 166 bp of OAS 1 gene exon 6(G166A),which contained three genotypes of GG,GA and AA.There was a SNP site at 206 bp of OAS 1 gene exon 6(A206G),which contained three genotypes of AA,AG and GG.The chi-square fitness test showed that G110C of OAS 1 gene exon 2 in Songliao Black pigs was in Hardy-Weinberg equilibrium,C176T,C145A,G166A and G206A in exons 3 and 6 deviated from Hardy-Weinberg equilibrium.The results of population genetic parameters analysis showed that the genetic heterozygosity of each SNP was in the middle level andmoderate polymorphism(0.25<PIC<0.5).The correlation analysis results showed that the total number of litter,live litter and weaning piglets of GC genotype in G110C were significantly higher than that of GG genotype(P<0.05).The number of weaned piglets of CT genotype in G176T was significantly higher than that of CC genotype(P<0.05).The total litter number and live number of CC genotype in C145T was significantly higher than that of AA genotype(P<0.05).The number of weaned piglets of GA genotype in G166A was significantly higher than that of GG genotype(P<0.05).The total number of offspring and live litter of GG genotype in A206G were significantly high

关 键 词：OAS 1基因 松辽黑猪 繁殖性状 多态性 关联分析 遗传标记 

分 类 号：S813.3[农业科学—畜牧学]