2013-2019年广西边境地区低致病性禽流感病毒监测分析  

Surveillance Analysis of Low Pathogenic Avian Influenza Virus in Border Areas of Guangxi from 2013 to 2019

作　　者：尹彦文[1] 施开创[1] 孙文超[2] 谢守玉 屈素洁[1] 陆文俊[1] 王露霞 覃勇 裴幸彪 凌丹 YIN Yanwen;SHI Kaichuang;SUN Wenchao;XIE Shouyu;QU Sujie;LU Wenjun;WANG Luxia;QIN Yong;PEI Xingbiao;LING Dan(Guangxi Center for Animal Disease Control and Prevention,Nanning 530001,China;Institute of Virology,Wenzhou University,Wenzhou 325035,China;Baise Center for Animal Disease Control and Prevention,Baise 533000,China;Fangcheng Center for Animal Disease Control and Prevention,Fangchenggang 530081,China;WumingCenter for Animal Disease Control and Prevention,Nanning 530199,China)

机构地区：[1]广西动物疫病预防控制中心,南宁530001 [2]温州大学病毒学研究所,温州325035 [3]百色市动物疫病预防控制中心,百色533000 [4]防城区动物疫病预防控制中心,防城港530081 [5]武鸣区动物疫病预防控制中心,南宁530199

出　　处：《中国畜牧兽医》2021年第8期3002-3009,共8页China Animal Husbandry & Veterinary Medicine

基　　金：广西自然科学基金项目(2020GXNSFBA297066);广西农业科技项目(Z201984,Z202032);兽医生物技术国家重点实验室开放课题(SKLVBF201610)。

摘　　要：为了解广西边境地区低致病性禽流感病毒(low pathogenic Avian influenza virus,LPAIV)的流行情况,本研究对2013-2019年在广西边境地区的规模养殖场和活禽市场采集的33964份家禽棉拭子样品,采用鸡胚接种方法进行病毒分离,并结合血凝抑制试验和RT-PCR等方法鉴定禽流感病毒的亚型。结果显示,共有3892份样品分离到LPAIV,2013-2019年的样品病毒分离率分别为17.39%、11.23%、13.59%、9.31%、9.09%、9.12%和15.21%,总分离率为11.46%,其中2013年样品的病毒分离率最高。分离到的LPAIV包括H1、H3、H4、H6、H9、H10和H117种亚型,样品病毒分离率分别为0.14%、4.37%、0.19%、4.06%、2.69%、0.01%和0.003%。其中活禽市场分离到H1、H3、H4、H6、H9、H10和H117种亚型流感病毒,样品病毒分离率分别为0.19%、5.53%、0.21%、5.29%、3.41%、0.01%和0.004%,总分离率为14.64%;而养殖场分离到H3、H4、H6和H94种亚型流感病毒,样品病毒分离率分别为1.26%、0.12%、0.79%和0.78%,总分离率为2.95%,养殖场的分离率远低于活禽市场(14.64%)。鸡源样品的病毒分离率为5.77%,水禽样品的病毒分离率为15.10%,环境样品的病毒分离率为21.67%,水禽和环境样品的病毒分离率远高于鸡。结果表明,当前广西边境地区家禽携带多种亚型LPAIV,水禽是高风险禽群,应进一步加强监测和防控;活禽市场污染严重,应进一步加强对活禽市场的监管力度,建立和实施更加科学有效的活禽市场运营机制。In order to understand the low pathogenic Avian influenza virus(LPAIV)epidemic situation in Guangxi border areas from 2013 to 2019,33964 poultry swabs samples were collected from large-scale farms and live poultry markets for LPAIV subtype isolation and identification through hemagglutination inhibition test and RT-PCR.The results showed that 3892 LPAIV strains were isolated,the virus isolation rates from 2013 to 2019 were 17.39%,11.23%,13.59%,9.31%,9.09%,9.12%and 15.21%,and the total isolation rate was 11.46%,of which the rate in 2013 was the highest.The isolated LPAIV included 7 subtypes H1,H3,H4,H6,H9,H10 and H11.These virus isolation rates were 0.14%,4.37%,0.19%,4.06%,2.69%,0.01%and 0.003%.In the live poultry market,LPAIV subtype H1,H3,H4,H6,H9,H10 and H11 rates were 0.19%,5.53%,0.21%,5.29%,3.41%,0.01%and 0.004%,respectively.While in the farms LPAIV subtype H3,H4,H6 and H9 rates were 1.26%,0.12%,0.79%and 0.78%,respectively.The virus isolation rate of the farm samples(2.95%)was obviously lower than the live poultry market samples(14.64%).The virus isolation rates of waterfowl(15.10%)and environmental samples(21.67%)were much higher than that from chickens(5.77%).The results showed that the current poultry carried multiple LPAIV subtypes,and waterfowl were high-risk flocks.The live poultry market was seriously polluted,and the market supervision(LPAIV)should be further strengthened,established and implemented more scientific,effective live poultry market operation mechanism.

关 键 词：低致病性禽流感病毒(LPAIV) 流行病学 广西边境地区 

分 类 号：S852.657[农业科学—基础兽医学]