Forensic investigation of 23 autosomal STRs and application in Han and Mongolia ethnic groups  

作　　者：Xiang Sheng Yali Wang Jiashuo Zhang Liqin Chen Yuan Lin Zhenmin Zhao Chengtao Li Suhua Zhang 

机构地区：[1]Shanghai Key Laboratory of Forensic Medicine,Shanghai Forensic Service Platform,Academy of Forensic Science,Shanghai,China [2]Department of Forensic Medicine,Medical College of Soochow University,Suzhou,China [3]Department of Forensic Medicine,Inner Mongolia Medical University,Hohhot,China

出　　处：《Forensic Sciences Research》2018年第2期138-144,共7页法庭科学研究（英文）

基　　金：This study was supported by grants from the National Key R&D Program of China[grant number 2016YFC0800703];the National Natural Science Fund of China[grant numbers 81625013 and 81772028];the Shanghai Technology Stan-dard Programme[grant number 16DZ0501600];the Shang-hai Key Laboratory of Forensic Medicine[grant number 17DZ2273200];the Shanghai Forensic Service Platform[grant number 16DZ2290900].

摘　　要：A forensic validation study of the Early Access HuaxiaTM Platinum Polymerase Chain Reaction (PCR) kit was completed to document the performance capabilities and limitations.The genotyping of DNA samples was consistent across a large range of template DNA concentrations,with complete profiles obtained at 0.125 ng;however,no more than 2 mm× 1.2 mm punches of samples would be recommended for direct amplification.The size precision and accuracy test revealed the genotyping ability;while consistent results were obtained when comparing the kit with other commercially available systems.In addition,the whole PCR amplification can finish within approximately 45 min,making the system suitable for fastdetection.However,only partial profiles may be obtained with challenging samples,including DNA stored on Foam-Tipped Applicators (FTA) cards or some case samples.For the forensic application in ethnic groups,a total of 282 and 229 alleles were obtained in Han and Mongolia,respectively.Since the 23 short tandem repeats were independent from each other,the cumulative power of exclusion in duos was 0.999999157188 and the cumulative power of exclusion in trios was 0.999999999859 in the Han group while the cumulative power of exclusion in duos (CPEduo) was 0.999 998 848 26 and cumulative power of exclusion in trios (CPEtrio) was 0.999 999 999 79 in the Mongolia group.And good internal consistency was found between the two investigated groups and the Sichuan Han,Hui,Tibetan and Uygur according to available reference data.

关 键 词：Forensic genetics short tandem repeats(STRs) Early Access HuaxiaTM Platinum PCR kit 

分 类 号：TP3[自动化与计算机技术—计算机科学与技术]