湖南桃江病圃稻瘟病菌生理小种鉴定及其毒性分析  

Identification of Physiological Races and Virulence Analysis of Magnaporthe oryzae from Rice Blast Nursery in Taojiang of Hunan Province

作　　者：李杰 李小娟 肖友伦 任佐华[1] 邓力喜[3] 夏新格 朱艳 刘二明[1] LI Jie;LI Xiao-juan;XIAO You-lun;REN Zuo-hua;DENG li-xi;XIA Xin-ge;ZHU Yan;LIU Er-ming(College of Plant Protection,Hunan Agricultural University,Hunan Changsha 410128,China;Hunan Plant Protection Institute,Hunan Changsha 410125,China;College of Biology and Agriculture,Zunyi Normal University,Guizhou Zunyi 563006,China;College of Agriculture,Hunan Agricultural University,Hunan Changsha 410128,China)

机构地区：[1]湖南农业大学植物保护学院,湖南长沙410128 [2]湖南省植物保护研究所,湖南长沙410125 [3]遵义师范学院生物与农业科技学院,贵州遵义563006 [4]湖南农业大学农学院,湖南长沙410128

出　　处：《西南农业学报》2021年第7期1451-1457,共7页Southwest China Journal of Agricultural Sciences

基　　金：湖南省农业科技创新资金项目(2016JC03,2018Q N14);贵州省教育厅自然科学研究项目(黔教合KY字[2015]413号)。

摘　　要：【目的】明确桃江病圃稻瘟病菌生理小种组成及其毒性,为湖南省水稻抗病品种的选育与利用提供参考依据。【方法】通过在番茄燕麦培养基(oat tomato agar medium,OTA)上诱导产孢,测定分离自桃江病圃的64个稻瘟病菌株的产孢能力,并利用水稻离体叶片接种技术鉴定分析其中53个菌株的生理小种类型及其毒性。【结果】2018年分离自桃江病圃的64个菌株中,诱导产孢后有53个菌株孢子量达1×10^(5)个/mL以上,满足离体接种对孢子浓度的要求;根据在7个中国鉴别品种上的抗感反应,53个菌株被划分为4群12个生理小种,优势种群为ZB,出现频率为79.2%,优势生理小种为ZB_(15),出现频率为39.6%;毒性较强的ZB_1小种出现频率仅次于ZB_(15),还出现了毒性最强的ZA_1小种。【结论】桃江病圃稻瘟病菌群体的毒性较强,优势生理小种为ZB_(15)。【Objective】Clarifying the composition of physiological races and virulence of Magnaporthe oryzae in rice blast nursery in Taojiang of Hunan province will provide a reference basis for utilization and development of rice varieties for resistance to rice blast disease in Hunan Province.【Method】The spore-generating ability of 64 rice blast isolates from Taojiang nursery was determined by inducing spore production on oat tomato agar medium(OTA),and the physiological races of 53 strains were identified and their virulence was further analyzed by using in vitro leaf inoculation technology.【Result】Among 64 isolates collected from Taojiang nursery in 2018,53 isolates could produce>1×10^(5)spores/mL after induced on OTA meidum,which met the requirement of in vitro leaf inoculation for spore concentration.Based on the reactions to seven Chinese differential varieties of rice,these isolates were divided into 4 groups and 12 physiological races,in which the dominant population was ZB with the frequency of 79.2%,and the dominant physiological race was ZB15 with the frequency of 39.6%.Furthermore,ZB1 had strong pathogenicity and the second highest frequency,and ZA1 possessing the strongest pathogenicity was also isolated from Taojiang nursery.【Conclusion】The population of rice blast fungus from Taojiang nursery is very strong in virulence to Chinese differential varieties of rice,in which the dominant physiological race is ZB_(15).

关 键 词：稻瘟病圃 稻瘟病菌 生理小种 病菌毒性 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]