低强度交变电磁场对于丙泊酚诱发老年大鼠认知功能衰退的治疗作用研究  

Effects of low-intensity alternating electromagnetic fields on propofol-induced cognitive function decline in aging rats

作　　者：高敏 刘文雄 GAO Min;LIU Wen-xiong(Department of Anesthesiology,Xidian Group Hospital of Xi'an,Xi'an 710077,China)

机构地区：[1]西安市西电集团医院麻醉科,西安710077

出　　处：《医疗卫生装备》2021年第8期7-11,25,共6页Chinese Medical Equipment Journal

摘　　要：目的:研究低强度交变电磁场(electromagnetic fields,EMF)治疗对于丙泊酚诱发老年大鼠认知功能衰退的作用及机制。方法:选取36只20月龄雄性Sprague-Dawley老年大鼠,随机分为丙泊酚组、丙泊酚+EMF组以及对照组(无丙泊酚及EMF干预),每组12只。丙泊酚组和丙泊酚+EMF组腹腔注射丙泊酚,首次注射剂量为60 mg/kg,随后每小时注射1次丙泊酚(注射剂量为30 mg/kg),共注射6次(即可维持麻醉状态6 h)。丙泊酚+EMF组大鼠每天进行6 h的全身EMF暴露(5 mT、50 Hz),连续7 d。使用水迷宫测试学习记忆功能,使用酶联免疫吸附测定法检测血清淀粉样β蛋白(Aβ)、血清神经元特异性烯醇化酶(NSE)、海马神经生长因子(NGF)及脑源性神经营养因子(BDNF)表达水平,分别使用TUNEL染色和蛋白质免疫印迹分析海马组织细胞凋亡水平及抗凋亡因子Bcl-2、凋亡标记物caspase-3表达水平。采用SPSS 20.0软件进行统计分析。结果:与丙泊酚组相比,丙泊酚+EMF组大鼠的逃逸潜伏期和游泳距离显著减少(P<0.05),穿越平台次数显著增加(P<0.05),血清Aβ和NSE表达水平显著降低(P<0.05),海马组织NGF和BDNF表达水平显著升高(P<0.05),海马凋亡细胞数量以及caspase-3的表达水平显著降低(P<0.05),Bcl-2表达水平显著升高(P<0.05)。丙泊酚+EMF组大鼠与对照组相比,在逃逸潜伏期、游泳距离、穿越平台次数以及Aβ、NSE、NGF、BDNF、Bcl-2、caspase-3表达水平方面均无统计学差异(P>0.05)。结论:低强度交变EMF能够显著改善丙泊酚诱发老年大鼠认知功能衰退症状,这与其对细胞凋亡的抑制以及Aβ、NSE、NGF和BDNF的调控相关。Objective:To study the effect and mechanism of low-intensity alternating electromagnetic field(EMF)on propofol-induced decline of the cognitive function in aging rats.Methods Thirty six 20-month-old male Sprague Dawley rats were randomly divided into a propofol group,a propofol+EMF group and a blank control group(with no propofol or EMF stimulation)with 12 rats in each group.In the propofol and propofol+EMF groups,propofol was injected intraperitoneally at an initial dose of 60 mg/kg,followed by one injection of propofol(at a dose of 30 mg/kg)every hour for a total of six injections(i.e.,to maintain anesthesia for 6 h).The rats in the propofol+EMF group were exposed to whole-body EMF(5 mT,50 Hz)for6 h daily for 7 d.Learning memory function was tested using the water maze,and serum amyloidβprotein(Aβ),serum neuronspecific enolase(NSE),hippocampal nerve growth factor(NGF)and brain-derived neurotrophic factor(BDNF)expression levels were measured using enzyme-linked immunosorbent assay.The levels of apoptosis,anti-apoptotic factor Bcl-2 and apoptotic marker caspase-3 in hippocampal tissue were analyzed by TUNEL staining and western blotting,respectively.SPSS 20.0 software was used for statistical analysis.Results As compared with the propofol group,the rats in the propofol+EMF group had lower escape latency and suimming distance(P<0.05),higher crossing platform times(P<0.05),significantly lower serum Aβand NSE expression levels(P<0.05),statistically higher hippocampal NGF and BDNF expression levels(P<0.05),lower number of apoptotic cells in hippocampus and caspase-3 expression level(P<0.05)and significantly higher Bcl-2 expression levels(P<0.05).There were no statistical differences in escape latency,swimming distance,times of crossing platform and expression levels of Aβ,NSE,NGF,BDNF,Bcl-2 and caspase-3 in the propofol+EMF group rats compared to the control group(P>0.05).Conclusion Low-intensity alternting EMF can significantly improve propofol-induced cognitive function decline in aging rats,which is associated

关 键 词：低强度交变电磁场 丙泊酚 认知功能 海马 细胞凋亡 

分 类 号：R318[医药卫生—生物医学工程] TM154[医药卫生—基础医学]