吸附伏安法对辅酶Q10的测定研究  

Study on Adsorptive Voltammetric Detection of Coenzyme Q10

作　　者：刘昱宏 郭斌 屠一锋[2] LIU Yu-hong;GUO Bin;TU Yi-feng(College of Pharmacy,Medical University of Jinzhou,Jinzhou 121000,China;College of Chemistry,Chemical Engineering and Material Science,Soochow University,Suzhou 215123,China)

机构地区：[1]锦州医科大学药学院,辽宁锦州121000 [2]苏州大学材料与化学化工学部,江苏苏州215123

出　　处：《分析测试学报》2021年第8期1224-1229,共6页Journal of Instrumental Analysis

摘　　要：该文研究了辅酶Q10在玻碳电极上的吸附伏安电化学行为,该吸附作用提供了提高测定灵敏度的途径。选择辅酶Q10溶解时的超声时间、测定温度、电解质溶剂的配比(甲醇∶乙醇)作为优化条件,使用响应面软件的Box-Behnken设计方案在单因素分析的基础上进行条件优化,所得条件为:辅酶Q10溶解的超声时间为1 min 50 s、测定温度为25℃、电解质溶剂配比为1.1∶1的甲醇-乙醇。上述优化条件下,经25 min吸附预富集,其伏安电流响应对辅酶Q10的测定线性范围为0.025~25.0μmol/L,线性方程为:I=2.554+1.479lg C,r^(2)=0.997,检出限(LOD)为19.0 nmol/L。该方法可实现辅酶Q10的精确、高响应定量分析。The electrochemical behavior of coenzyme Q10(CoQ10)on glassy carbon electrode(GCE)was explored by cyclic voltammetry(CV)in this paper.The results revealed the adsorption of CoQ10 on GCE,thus providing an approach to enhance the sensitivity for its detection by pre-enrichment.The experimental conditions including ultrasonic duration for CoQ10 extraction,detection temperature and the ratio of electrolyte solvent(methanol-ethanol)were optimized by Box-Behnken method as 1 min 50 s,25℃and 1.1∶1,respectively.Under the optimized conditions,after 25 min of adsorption,the CV current linearly responded.to the logarithm of CoQ10 concentration within the range of 0.025-25.0μmol/L,with a regression equation of I=2.554+1.479lg C(r^(2)=0.997),and a detection limit(LOD)of 19.0 nmol/L.The detection of CoQ10 by using adsorptive CV on GCE could achieve its accurate and high response quantitative analysis.

关 键 词：辅酶Q10 吸附伏安法 响应面优化 定量分析 

分 类 号：O657.1[理学—分析化学] O629.8[理学—化学]