3D打印矿化胶原基骨修复体降解及其生物学性能研究  

Study on the Degradation and Biological Properties of 3D Printing Mineralized Collagen-Based Bone Repair Materials

作　　者：胡堃[1,2,3,4,5] 王坤兰 崔玉珠 肖永昊 杨桂娟 孙玮玮 王峻东 王海波 郭林鑫峥 韩璐 李路海[1,2] 莫黎昕[1,2] 杨永刚[1,2] 邓普君[1,2] 马天一[1,2] 危岩[5,6] HU Kun;WANG Kun-lan;CUI Yu-zhu;XIAO Yong-hao;YANG Gui-juan;SUN Wei-wei;WANG Jun-dong;WANG Hai-bo;GUO Lin-xinzheng;HAN Lu;LI Lu-hai;MO Li-xin;YANG Yong-gang;DENG Pu-jun;MA Tian-yi;WEI Yen(School of Printing and Packaging Engineering,Beijing Institute of Graphic Communication,Beijing 102600,China;Beijing Engineering Research Center of Printed Electronics,Beijing Institute of Graphic Communication,Beijing 102600,China;Bioprinting Engineering Technology Center,Beijing Institute of Graphic Communication,Beijing 102600,China;College of Biological Science and Engineering,Fuzhou University,Fuzhou 350108,China;Department of Chemistry,Tsinghua University,Beijing 100084,China;Frontier Polymer Research Center,Tsinghua University,Beijing 100084,China)

机构地区：[1]北京印刷学院印刷与包装工程学院,北京102600 [2]北京印刷学院北京印刷电子工程技术研究中心,北京102600 [3]北京印刷学院生物印刷工程技术中心,北京102600 [4]福州大学生物科学与工程学院,福州350108 [5]清华大学化学系,北京100084 [6]清华大学前沿高分子研究中心,北京100084

出　　处：《数字印刷》2021年第3期147-157,共11页Digital Printing

基　　金：北京印刷学院校级项目--普通重点教学改革项目--基于OBE教育模式的大学生创新创业能力提升研究--《高分子科学进展II》课程为例(No.22150121033/006);3D打印及在生物医学中的应用(No.11000300653);3D打印定制化骨材料及模具设计制备(No.11000300880);3D打印教育模型的制备(No.11000300897);创新人才培养北京五中化学基础翱翔学员培养与课程开发(No.11000301026)。

摘　　要：本研究将仿生学方法制备的纳米晶矿化胶原(nHAC)与聚乳酸(PLA)复合制备原材料,用于熔融挤压堆积成型(FDM)法制备骨修复体。采用碱性磷酸酶(ALP)试剂盒检测细胞ALP活性,扫描电镜观察细胞与材料间的相互作用,并观察到细胞出现黏附增殖。在动物实验中,植入个性化的仿生骨修复体,结果显示复合骨修复体逐渐降解并被新长出的骨组织所取代。实验表明了3D打印的骨修复体及制备的nHAC/PLA复合线材降解性能好,具备良好的细胞黏附和细胞相容性且细胞毒性较小,并能促进成骨细胞的增殖,从而促进骨愈合。In this study,the nanocrystalline mineralized collagen(nHAC)prepared by the biomimetic method was combined with polylactic acid(PLA)to prepare the raw material which was used to prepare bone repair materials with the fused deposition modeling(FDM)method.The alkaline phosphatase(ALP)kit was used to detect the ALP activity of the cells,the scanning electron microscope was used to observe the interaction between the cells and the materials,and the adhesion and proliferation of the cells were observed.In animal experiments,personalized bionic bone repair materials were implanted,and the results showed that the composite bone repair materials gradually degrade and can be replaced by newly grown bone tissue.Experiments have shown that the three-dimensional printed bone repair material and the prepared nHAC/PLA composite wire have good degradation performance,good cell adhesion and cytocompatibility,low cytotoxicity,and can promote the proliferation of osteoblasts,thereby promoting bone healing.

关 键 词：纳米晶矿化胶原 聚乳酸 3D打印 细胞毒性 成骨性能 

分 类 号：R318.08[医药卫生—生物医学工程]