IGF-1和IGF-2基因在关岭牛不同组织中的表达分析  

Expression analysis of IGF-1 and IGF-2 Genes in Different Tissues of Guanling Cattle

作　　者：付开斌 陈祥[1,2] 吴雨 周志楠 张艳[1,2] 惠茂茂[1,2] FU Kaibin;CHEN Xiang;WU Yu;ZHOU Zhinan;ZHANG Yan;HUI Maomao(Key Laboratory of Genetic Breeding and Reproduction of Plateau and Mountain Animals of the Ministry of Education,Guizhou Key Laboratory of Animal Genetic Breeding and Reproduction,Guizhou University,Guizhou Guiyang 550025,China;College of Animal Science,Guizhou University,Guizhou Guiyang 550025,China)

机构地区：[1]贵州大学高原山地动物遗传育种与繁殖教育部重点实验室,贵州省动物遗传育种与繁殖重点实验室,贵州贵阳550025 [2]贵州大学动物科学学院,贵州贵阳550025

出　　处：《中国畜牧杂志》2021年第8期72-76,共5页Chinese Journal of Animal Science

基　　金：贵州省农业领域重点项目(黔科合NY[2015]3002号)。

摘　　要：为探究IGF-1基因和IGF-2基因在关岭牛不同组织中的表达规律,本实验以关岭牛为研究对象,采集关岭牛的肝脏、脾脏、心脏、肺脏、肾脏以及背最长肌6个不同组织,提取总RNA,并采用qRT-PCR技术分析IGF-1基因和IGF-2基因mRNA在关岭牛6个不同组织中的表达水平。结果显示:IGF-1基因在关岭牛6个不同组织中均有表达,与其他5个组织相比,肺脏表达量极显著高于所有组织,IGF-1基因在背最长肌的表达量低于其他5个组织,肝脏的表达量显著高于背最长肌,其余各组织间均无显著差异;IGF-2基因在关岭牛6个不同组织中均有表达,在肺脏中的表达量极显著高于其他5个组织,其次是肝脏,最低为背最长肌,在肝脏组织中的表达量显著高于脾脏和背最长肌,其余组织间无显著差异。本研究揭示了IGF-1基因和IGF-2基因在关岭牛中的表达差异性,为探究关岭牛的生长发育机制提供了基础数据。In order to study the expression levels of IGF-1 and IGF-2 genes in different tissues of Guanling cattle,six different tissues of liver,spleen,heart,lung,kidney and longissimus dorsi were collected from Guanling cattle.The total RNA was extracted and the expression levels of IGF-1 gene and IGF-2 gene mRNA in six different tissues of Guanling cattle were analyzed by qRT-PCR.The results showed that IGF-1 gene was expressed in six different tissues of Guanling cattle.Compared with the other five tissues,the expression of IGF-1 gene in lung was significantly higher than that in all tissues,the expression of IGF-1 gene in longissimus dorsi was lower than that in other five tissues,and the expression in liver was significantly higher than that in longissimus dorsi,but there was no significant difference among other tissues.IGF-2 gene was expressed in six different tissues of Guanling cattle,the expression in lung was significantly higher than that in other five tissues,the second was liver,the lowest was longissimus dorsi,and the expression in liver was significantly higher than that in spleen and longissimus dorsi,but there was no significant difference among other tissues.This study revealed the difference of expression of IGF-1 gene and IGF-2 gene in Guanling cattle,which provided basic data for exploring the growth and development mechanism of Guanling cattle.

关 键 词：关岭牛 荧光定量PCR 表达量 IGF-1 IGF-2 

分 类 号：S823.2[农业科学—畜牧学]