金华猪肠道ANGPTL4的表达分布特征和发育性变化研究  

Study on the Expression and Distribution Characteristics and Developmental Changes of Angiogenin-like Protein 4 in the Intestinal Tract of Jinhua Pig

作　　者：郑自彬 吕文涛 章啸君 任莹[2] 项云 杨华 肖英平 ZHENG Zibin;LYU Wentao;ZHANG Xiaojun;REN Ying;XIANG Yun;YANG Hua;XIAO Yingping(State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Institute of Agro-product Safety and Nutrition,Zhejiang Academy of Agricultural Sciences,Zhengjiang Hangzhou 310021,China;Hubei Key Laboratory of Animal Nutrition and Feed Science,Wuhan Polytechnic University,Hubei Wuhan,430023,China;Institute of Animal Husbandry and Veterinary Medicine,Jinhua Academy of Agricultural Sciences,Zheng jiang Jinhua 321070,China)

机构地区：[1]省部共建农产品质量安全危害因子与风险防控国家重点实验室,浙江农业科学院农产品质量安全与营养研究所,浙江杭州310021 [2]武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉430023 [3]金华市农业科学研究院畜牧兽医研究所,浙江金华321017

出　　处：《中国畜牧杂志》2021年第8期87-91,共5页Chinese Journal of Animal Science

基　　金：国家自然科学基金(31972999)。

摘　　要：血管生成素样蛋白4(ANGPTL4)在机体脂肪和能量代谢中起重要的调控作用,并参与介导肠道微生物区系对脂肪沉积的调节。本实验以金华猪为研究对象,分析ANGPTL4在猪肠道中的分布特征及其发育性变化。分别选取45、90、150日龄和270日龄体重相近的健康金华猪各4头,取十二指肠、空肠、回肠、盲肠和结肠等组织,通过RT-PCR测定ANGPTL4基因的相对表达量;同时取270日龄金华猪肠道组织进行免疫组织化学分析,观察ANGPTL4在肠道中的染色情况。结果表明:免疫组化显示在金华猪的十二指肠、空肠、回肠、结肠和盲肠等组织中均有ANGPTL4阳性细胞分布;金华猪空肠ANGPTL4表达量显著高于十二指肠和结肠;在90日龄阶段金华猪空肠、回肠和结肠的ANGPTL4相对表达量均显著高于270日龄,且十二指肠和盲肠ANGPTL4相对表达量在90日龄达到最高,随后逐渐降低。综上,ANGPTL4在金华猪十二指肠、空肠、回肠、结肠和盲肠等组织中均有分布,在空肠中的表达量相对较高;同时在90日龄后,随日龄增加,金华猪各肠道ANGPTL4表达量均呈现下降趋势。Angiopoietin like protein 4(ANGPTL4)plays an important regulatory role in the body's fat and energy metabolism,and participates in the regulation of intestinal microflora on fat deposition.In this study,Jinhua Pig was used as the research object to analyze the distribution characteristics and developmental changes of ANGPTL4 in the intestinal tract of pigs.Four healthy Jinhua Pigs with similar body weight at 45,90,150 and 270 days of age were selected,respectively.The tissues of duodenum,jejunum,ileum,cecum and colon were collected to determine the gene expression of ANGPTL4 by RT-PCR.Meanwhile,the intestinal tissues of 270-day-old Jinhua pigs were taken for immunohistochemical analysis to observe the staining of ANGPTL4 in the intestine.The results showed that:immunohistochemical staining showed that ANGPTL4 positive cells were distributed in duodenum,jejunum,ileum,colon,and cecum of Jinhua Pigs;RT-PCR analysis showed that the expression of ANGPTL4 in jejunum was significantly higher than that in duodenum(P=0.025)and colon(P=0.047);At 90 days of age,the relative expression levels of ANGPTL4 in jejunum,ileum and colon of Jinhua pigs were significantly higher than those of 270 days old,and the relative expression levels of ANGPTL4 in duodenum and cecum reached the highest at 90 days old,and then decreased gradually.The results of immunohistochemistry and RT-PCR showed that ANGPTL4 was distributed in duodenum,jejunum,ileum,colon and cecum of Jinhua pigs,and the expression level of ANGPTL4 in jejunum was much higher than that in duodenum and colon.Additionally,the expression of ANGPTL4 in each intestine of Jinhua Pig showed a downward trend with the increase of age.

关 键 词：ANGPTL4 金华猪 肠道 发育性变化 

分 类 号：S828.2[农业科学—畜牧学]