产DHA破囊壶菌Aurantiochytrium sp.HX2019010的筛选及其脂肪酸组成分析  

Screening of DHA-Producing Thraustochytrium Roseum Aurantochytrium sp.HX2019010 and Analysis of Its Fatty Acid Composition

作　　者：肖玉娟 甘美裕 林俊杰 庄峙厦 何少贵 傅奇 Xiao Yujuan;Gan Meiyu;Lin Junjie;Zhuang Zhixia;He Shaogui;Fu Qi(College of Environment and Public Health,Xiamen Huaxia University,Xiamen 361024)

机构地区：[1]厦门华厦学院环境与公共健康学院,厦门361024

出　　处：《中国粮油学报》2021年第6期81-86,共6页Journal of the Chinese Cereals and Oils Association

基　　金：福建省大学生创新创业训练计划(202012709006,202012709028);生化制药福建省高校工程研究中心开放课题(SHZY202001);福建省中青年教师教育科研项目(JT180782);厦门市高校科研院所产学研切块管理项目(3502Z20193092)。

摘　　要：为筛选产二十二碳六烯酸(Docosahexaenoic acid,DHA)菌株,从厦门市红树林的海水腐木中分离到一株球状真菌HX2019010,通过扫描电镜观察,测定ITS序列,与Genbank数据库比对,该菌株与破囊壶菌相似性较高。选取ITS序列相似性较高的相近菌株构建的进化树表明,菌株HX2019010与Aurantiochytrium类群聚为一枝,自举检验值较高,初步定名为Aurantiochytrium sp.HX2019010。该菌株在发酵罐中可产生较高浓度的DHA,98 h发酵后生物量达108.27 g/L,总油脂达28.43 g/L,DHA达9.17 g/L。细胞脂肪酸组成分析结果表明,该菌株所产脂肪酸主要为棕榈酸、DHA、肉豆蔻酸等;脂肪酸组成受培养时间或培养条件的影响,总量可达细胞干质量的26.26%,其中DHA占脂肪酸总量的32.25%-45.86%。In order to screen docosahexaenoic acid(DHA)producing strain,a spherical fungus from the sea rot wood of mangroves in Xiamen City was isolated,which preliminarily identified to be Aurantiochytrium sp.HX2019010,for strain HX2019010 and other Aurantiochytrium strains were clustered together in phylogenetic tree based on ITS sequences from Genbank,and cell morphology observed by scanning electron microscopy wasalso similar to that of Aurantochytrium.The strain HX2019010 couldproduce considerable concentration of DHA in the bioreactor.After 98 hours of fermentation,the amount of biomass reached 108.27 g/L,the total fat reached 28.43 g/L,and the DHA reached 9.17 g/L.The fatty acids produced by strain HX2019010 were mainly palmitic acid,DHA andmyristic acid,etc.,and the composition of fatty acid was affected by the culture time or conditions.The high level of total fat was 26.26%of the dry cell weight,and the proportion of DHA in fatty acids was 32.25%~45.86%.

关 键 词：破囊壶菌 DHA 鉴定 脂肪酸 

分 类 号：Q939.97[生物学—微生物学]