烟叶稳定碳同位素组成受控因素分析  

Analysis of Controlled Factors of Stable Carbon Isotope Composition in Tobacco Leaves

作　　者：毛佳琦 郑允允 张二强 王培 付玉洁 王瑶[2] MAO Jiaqi;ZHENG Yunyun;ZHANG Erqiang;WANG Pei;FU Yujie;WANG Yao(Zhongyuan Instute,Zhejiang University,Zhengzhou 450001,China;Technology Center,China Tobacco Shanxi Industiral co.,ltd,Baoji 721013,China)

机构地区：[1]浙江大学中原研究院,河南郑州450001 [2]陕西中烟工业有限责任公司技术中心,陕西宝鸡721013

出　　处：《广东农业科学》2021年第7期17-23,共7页Guangdong Agricultural Sciences

基　　金：陕西中烟工业有限责任公司卷烟制丝大数据分析与智能化应用技术研究项目(BA000-ZB19015)。

摘　　要：【目的】探讨烟叶稳定碳同位素组成的受控因素,为烟草种植评价指标体系的完善及生态适应性机理研究提供新的理论依据。【方法】选取我国西南、东南、长江中上游和黄淮4个主要一级烟草种植区生理成熟期烟叶进行稳定碳同位素组成(δ13C)分析。【结果】供试烟叶样本δ13C值分布在-27.76‰--25.39‰,平均值为-26.46‰。烟叶δ13C值与经度呈显著负相关关系,经度每增加1°,烟叶δ13C值减小0.09‰;与纬度呈二次抛物线关系。不同叶位烟叶δ13C值具有显著差异,表现为上部叶片>中部叶片>下部叶片。4个种植区烟叶δ13C值为西南种植区≈黄淮种植区>长江中上游>东南种植区。利用碳同位素对不同香型烟叶聚类结果表明,不同产地的清香甜香型和醇甜香型烤烟能够被明确聚类,而对焦甜焦香型、清甜蜜甜香型、焦甜醇甜香型和蜜甜香型4种香型烤烟没有明显的聚类效果。【结论】烟叶稳定碳同位素组成与经度呈负相关关系,与纬度呈二次抛物线关系,不同叶位烟叶稳定碳同位素组成不同,利用δ13C值可以对清香甜香型和醇甜香型烤烟进行有效聚类。【Objective】The study was conducted to explore the controlled factors of the stable carbon isotope composition in tobacco leaves with a view to provide a new theoretical basis for the improvement of tobacco planting evaluation index system and the study of ecological adaptability mechanism.【Method】The stable carbon isotope composition(δ13C)analysis was carried out on tobacco leaves which were collected during the mature stage from four major first class tobacco growing areas in China,namely,Southwest China,Southeast China,upper and middle reaches of the Yangtze River and Huanghuai area.【Result】Theδ13C values of tobacco leaves ranged from-27.76‰to 25.39‰,with an average value of-26.46‰.Theδ13C values decreased with longitude significantly,with a rate of 0.09‰,and the values showed a quadratic parabola relationship with latitude.In addition,there were significant differences inδ13C values in different leaf positions,and the results showed as upper leaves>middle leaves>lower leaves.In terms of tobacco growing areas,δ13C value results of four tobacco growing areas showed as:Southwest planting area≈Huanghuai planting area>Yangtze River planting area>Southeast planting area.Moreover,the cluster analysis results showed that fragrant sweet tobacco and alcoholic sweet aroma tobacco were clustered clearly based onδ13C values of tobacco from different origins.However,the other four aroma types(burnt-sweet and burnt-fragrant type,mildly and honey sweet type,burnt-sweet and alcoholic sweet type and honey sweet type)cannot be clustered obviously as the independent variables.【Conclusion】The stable carbon isotope composition of tobacco leaves has a negative correlation with longitude and a quadratic parabolic relationship with latitude.The stable carbon isotope composition of tobacco leaves at different leaf positions is different.Theδ13C value can be used to effectively cluster the fragrant sweet and alcoholic sweet aroma tobacco.

关 键 词：烟叶 叶位 经度 纬度 稳定碳同位素 聚类分析 

分 类 号：S572[农业科学—烟草工业] Q945.79[农业科学—作物学]