纸喷雾软电离质谱分析性能研究  

Exploration of the Soft Ionization Performance of Paper Spray Mass Spectrometry

作　　者：许泽汝 赵佳 郑亚君[1] 牛志逢 向志成 张智平[1] XU Ze-ru;ZHAO Jia;ZHENG Ya-jun;NIU Zhi-feng;XIANG Zhi-cheng;ZHANG Zhi-ping(School of Chemistry and Chemical Engineering,Ni'an Shiyou University,Xi'an 710065,China)

机构地区：[1]西安石油大学化学化工学院,陕西西安710065

出　　处：《质谱学报》2021年第4期390-399,I0003,共11页Journal of Chinese Mass Spectrometry Society

基　　金：国家自然科学基金(21777128);陕西省自然科学基础研究计划项目(2019JC-33);陕西高校青年创新团队项目(Z19257)。

摘　　要：纸喷雾电离源-质谱法具有操作简单、价格低廉、无需繁琐的预处理便可对复杂样品进行快速分析等特点。作为一种电喷雾离子源,关于纸喷雾电离源与常用电喷雾电离源、纳升电喷雾电离源之间离子化性能的差异鲜有报道。本文采用汽油、肌红蛋白、细胞色素c和GAP样品作为分析对象,系统地比较了上述3种电离源的离子化特征,发现电离程度呈现纸喷雾电离源<电喷雾电离源<纳升电喷雾电离源的趋势,其中纸喷雾电离源表现出最软的电离特性。该研究对于深入了解不同电离源性能之间的差异,拓展纸喷雾电离源的应用范围具有参考价值。Paper spray mass spectrometry has received considerable attentions in recent years due to its simple operation, low cost and without tedious sample pretreatment prior to analysis. However, paper spray ionization(PSI) as a type of electrospray-based ionization source, there are few reports on exploring the ionization performance differences between it and two frequently used electrospray ionization(ESI) and nanoelectrospray ionization(nanoESI). Herein the ionization performance of above three ionization sources was compared systematically by using gasolines, myoglobin, cytochrome c and glycidyl azide polymer(GAP) as samples. According to the obtained results, the ionization performance demonstrated the following trend: PSI<ESI<nanoESI, in which PSI gave the softest ionization performance relative to other two ionization sources. Specifically, when commercially available 92# and 95#(Research Octane Number, RON) gasolines were used for analysis, PSI was favorable to give more intensive peaks for stronger polar compounds, whereas more abundant peaks for weaker polar compounds were observed in mass spectra by ESI and nanoESI. When proteins such as myoglobin and cytochrome c were ionized, the charge state distribution(CSD) for PSI moved to higher mass-to-charge ratios relative to ESI and nanoESI, and the average charge states(Zave) from PSI were lower than those from ESI and nanoESI. As an example for myoglobin, the Zave value was 18.07±0.01 for PSI, whereas the corresponding values were 19.47±0.01(ESI) and 19.67±0.01(nanoESI) in comparable experimental conditions(n=3), respectively. When cytochrome c was used for analysis, the Zave values were 14.14±0.17(PSI), 14.44±0.13(ESI) and 14.67±0.01(nanoESI), respectively. As GAP sample was analyzed using PSI, it was more favorable to maintain the structures of intact GAP, and both the number-average molecular weight(Mn) and weight-average molecular weight(Mw) had higher values. For both ESI and nanoESI, lower values of Mn and Mw were obtained. These results provided con

关 键 词：纸喷雾 电喷雾 质谱分析 电离性质 软电离 

分 类 号：O657.63[理学—分析化学]