基于代谢组学的黄芩苷、盐酸小檗碱抑制鲍曼不动杆菌作用机制的初步研究  

Preliminary Study on Mechanism of Baicalin and Berberine Hydrochloride in Inhibiting Acinetobacter Baumannii Based on Metabolomics

作　　者：倪建腾[1] 马致洁[1] 赵奎君[1] 章从恩[1] 陈熹 NI Jianteng;MA Zhijie;ZHAO Kuijun;ZHANG Congen;CHEN Xi(Dept.of Traditional Chinese Medicine Pharmacy,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院中药剂科,北京100050

出　　处：《中国医院用药评价与分析》2021年第7期834-841,共8页Evaluation and Analysis of Drug-use in Hospitals of China

摘　　要：目的:利用代谢组学对黄芩苷、盐酸小檗碱体外抑制鲍曼不动杆菌的作用机制进行初步探讨,为临床用药提供新思路。方法:采用液相色谱-质谱联用技术分析代谢谱,将鲍曼不动杆菌胞外代谢物的中性离子数据进行主成分分析(PCA)与正交偏最小二乘法-判别分析(OPLS-DA),依据Metlin数据库初步筛选差异代谢物,结合文献并使用MetaboAnalyst在线软件对差异代谢物进行代谢通路富集分析。结果:初步筛选出L-半胱氨酸(黄芩苷组)和尿苷三磷酸(盐酸小檗碱组)等差异代谢物;与对照组相比,L-半胱氨酸在黄芩苷组,尿苷三磷酸含量在盐酸小檗碱组中有显著变化,差异均有统计学意义(P<0.05)。代谢通路富集分析结果显示,谷氨酸代谢通路、半胱氨酸代谢通路等与黄芩苷作用机制相关;嘧啶代谢通路、氨基糖代谢通路等与盐酸小檗碱作用机制相关。结论:黄芩苷可能通过调节谷氨酸、半胱氨酸等代谢通路抑制鲍曼不动杆菌,盐酸小檗碱可能通过调节嘧啶代谢、氨基糖代谢等通路抑制鲍曼不动杆菌。OBJECTIVE:To preliminarily explore the mechanism of baicalin and berberine hydrochloride in inhibiting Acinetobacter baumannii(A.baumannii)in vitro based on metabonomics,so as to provide new ideas for clinical medication.METHODS:The The metabolites were analyzed by liquid chromatography-mass spectrometry,and the neutral ion data of the extracellular metabolites of A.baumannii were subjected to principal component analysis(PCA)with orthogonal partial least squares-discriminant analysis(OPLS-DA).Metlin database was used to determine the differential metabolites,combined with the literature and MetaboAnalyst online software,the metabolic pathway enrichment analysis of the differential metabolites was performed.RESULTS:Differential metabolites such as L-cysteine(baicalin group)and uridine triphosphate(berberine hydrochloride group)were preliminarily screened out.Compared with the control group,the content of L-cysteine in the baicalin group and the content of uridine triphosphate in the berberine hydrochloride group had significant changes,and the differences were statistically significant(P<0.05).Metabolic pathway enrichment analysis showed that glutamate metabolism pathway and cysteine metabolism pathway were related to the mechanism of baicalin;pyrimidine metabolism pathway and amino sugar metabolism pathway were related to the mechanism of berberine hydrochloride.CONCLUSIONS:Baicalin may inhibit A.baumannii by regulating metabolic pathways such as glutamate and cysteine,while berberine hydrochloride may inhibit A.baumannii by regulating pathways such as pyrimidine metabolism and amino sugar metabolism.

关 键 词：鲍曼不动杆菌 黄芩苷 盐酸小檗碱 代谢机制 

分 类 号：R96[医药卫生—药理学]