水稻稻瘟病生防细菌5-8的鉴定及其生防机制研究  

Identification of rice blast control bacteria 5-8 and its biocontrol mechanism

作　　者：李珅瑀 贾祥子 郭君陶 张清霞[1] 陈夕军[1] LI Shenyu;JIA Xiangzi;GUO Juntao;ZHANG Qingxia;CHEN Xijun(College of Horticulture and Plant Protection,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学园艺与植物保护学院,江苏扬州225009

出　　处：《北方农业学报》2021年第3期74-81,共8页Journal of Northern Agriculture

基　　金：江苏省重点研发计划项目(BE2017344,BE2018359);国家自然科学基金项目(31772210,32072471);扬州市市级计划——市校合作专项(YZ2018137)。

摘　　要：【目的】获得对水稻稻瘟病有较好生防效果的拮抗细菌并探索其作用机理。【方法】采用苗期防病试验,从保存的10种拮抗细菌中筛选出对水稻稻瘟病有较好防治效果的菌株5-8。通过对峙培养测定其抑菌谱。结合生理生化指标和16S rDNA基因序列同源性分析,鉴定该细菌分类地位,并通过PCR扩增、MALDI-TOF MS检测鉴定菌株5-8产生的抗菌物质种类。【结果】菌株5-8对水稻稻瘟病的防病效果最好,防效可达90%以上;该细菌抑菌谱广,对油菜菌核病菌、桃褐腐病菌等均有较强的抑菌活性。16S rDNA基因序列同源性分析和生理生化指标测定结果表明,菌株5-8为贝莱斯芽孢杆菌(Bacillus velezensis);PCR可扩增到丰原素、伊枯草菌素,并结合MALDI-TOF MS的结果初步判定菌株5-8可产生伊枯草菌素。【结论】菌株5-8被鉴定为贝莱斯芽孢杆菌(Bacillus velezensis),主要通过产生脂肽类抗生素伊枯草菌素防治水稻稻瘟病。【Objective】To obtain antagonistic bacteria with good biocontrol effect on rice blast and explore its control mechanism.【Methods】The strain 5-8 with better control effect on rice blast was selected from 10 kinds of antagonistic bacteria in seedling stage.The bacteriostatic spectrum was determined by confrontation culture,and the taxonomic status was identified by physiological and biochemical indexes and 16S rDNA gene sequence homology analysis.The antibacterial substances produced by strain 5-8 were identified by PCR amplification and MALDI-TOF MS.【Results】Strain 5-8 had the best control effect on rice blast,and the control effect could reach more than 90%,and it had a wide antibacterial spectrum and strong antibacterial activity against Sclerotinia sclerotiorum and Monilinia fructicola.16S rDNA gene sequence homology analysis and physiological and biochemical indexes measurement results showed that strain 5-8 was Bacillus velezensis;the results of PCR amplification of progenin and ituron,combined with MALDI-TOF MS,preliminarily determined that strain 5-8 could produce ituron.【Conclusion】The strain 5-8 was identified as Bacillus velezensis,which mainly control of rice blast by producing lipopeptide antibiotic ituron.

关 键 词：水稻稻瘟病 菌株5-8 贝莱斯芽孢杆菌 伊枯草菌素 生物防治 

分 类 号：S511[农业科学—作物学]