透皮活血中药垫枕枕芯对下肢深静脉血栓形成犬模型的安全性和有效性  

Safety and effectiveness of lower limb deep venous thrombosis transdermal blood-activating traditional Chinese medicine bolster pillow inner through thrombosis models

作　　者：雍熙 康藤耀 陈开[1] 王贤芝 张富钊 宁俊洁 任逢春 杨勤[5] 尹洪顺 陈镜全 郑江华 YONG Xi;KANG Teng-yao;CHEN Kai;WANG Xian-zhi;ZHANG Fu-zhao;NING Jun-jie;REN Feng-chun;YANG Qin;YIN Hong-shun;CHEN Jing-quan;ZHENG Jiang-hua(Department of Vascular Surgery,Affiliated Hospital of North Sichuan Medical College,Nanchong 637000;Department of Cardiothoracic Vascular Surgery,the First People s Hospital of Guangyuan,Guangyuan 628000;Beichuan Qiang Autonomous County People’s Hospital,Mianyang 622650;Department of Radiology,Affiliated Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610000;Department of Infectious Diseases,Affiliated Hospital of North Sichuan Medical College,Nanchong 637000;Department of Clinical Medicine,North Sichuan Medical College,Nanchong 637000,Sichuan,China)

机构地区：[1]川北医学院附属医院血管外科,四川南充637000 [2]广元市第一人民医院心胸血管外科,四川广元628000 [3]北川羌族自治县人民医院,四川绵阳622650 [4]成都中医药大学附属医院放射科,四川成都610000 [5]川北医学院附属医院感染科 [6]川北医学院临床医学系,四川南充637000

出　　处：《川北医学院学报》2021年第8期959-963,共5页Journal of North Sichuan Medical College

基　　金：国家自然科学基金(81370441);白求恩医学科学研究基金(AE004CS);川北医学院科研发展计划重点项目(CBY16-A-ZD09);四川省南充市2018年市校战略合作科技项目(18SXHZ0382)。

摘　　要：目的:探究透皮活血中药垫枕枕芯对试验犬下肢深静脉血栓模型的安全性及有效性。方法:构建深静脉血栓比格犬模型,并随机分为实验组(透皮活血中药)和空白对照组,每组8只,分别于治疗前和治疗后1、2、4、12、16周抽血比较两组试验动物的D-二聚体、血常规、凝血功能及肝肾功等指标,并行彩超检查了解下肢血栓再通、复发情况;治疗后16周处死试验动物,取心、肝、肺、脑和肾脏标本行病理学检查。结果:两组试验动物进食正常、活动正常,血常规、凝血功能、肝肾功、电解质(血钾、钙、钠)在治疗后1、2、4、12、16周比较,差异无统计学意义(P>0.05);两组D-二聚体在治疗后1、2周比较,差异无统计学意义(P>0.05),但在治疗后4、12、16周,实验组的D-二聚体水平低于对照组,差异有统计学意义(P<0.05);治疗后16周内,两组心、肝、肺、肾、脑病理检查均未见异常;治疗后16周内,实验组的血栓复发率与对照组比较,差异无统计学意义(P>0.05),且实验组血栓完全再通,而对照组血栓部分再通。结论:透皮活血中药垫枕枕芯用于下肢深静脉血栓形成动物犬模型后,对血液常规、生化和重要脏器结构功能无明显影响,安全性较高,且可轻度降低血D-二聚体,降低血栓远期复发率,具有一定的临床应用前景及价值。Objective:To investigate the safety and effectiveness of lower limb deep venous thrombosis transdermal blood-activating traditional Chinese medicine bolster pillow inner through thrombosis models.Methods:Beagle dogs with deep vein thrombosis were randomly divided into experimental group(transdermal Chinese Medicine)and blank control group,8 dogs in each group.Blood samples were collected to measure D-dimer,blood routine,blood coagulation function,liver and kidney functions,and electrolyte before treatment and 1,2,4,12 and 16 weeks after treatment.Colorful ultrasound dopplar were performed to investigate thrombus recanalization and reoccurrence.All animals were sacrificed in the 16 th week,and the tissue of heart,liver,lung,brain and kidney were subjected to pathological examination.Results:The animals in the two groups ate normally and moved normally,there was no significant difference in blood routine,coagulation function,liver and kidney function and electrolytes(blood potassium,calcium and sodium)between the two groups at 1,2,4,12 and 16 weeks after treatment(P>0.05).There was no significant difference in D-dimer between the two groups at 1 and 2 weeks after treatment(P>0.05),the plasma levels of D-dimer in the experimental group was lower than that in the control group at 4,12 and 16 weeks(P<0.05).Within 16 weeks after treatment,no abnormality was found in the heart,liver,lung,kidney and brain of the two groups.The recurrence rate of thrombosis in the experimental group was lower than that in the control group(0.00%vs.25.00%),the difference was not statistically significant(P>0.05).Thrombus of the experimental groups was all completely recanalized,and the thrombus of the control group was partially recanalized.Conclusion:The application of lower limb deep venous thrombosis transdermal blood-activating traditional Chinese medicine bolster pillow inner has no obvious effect on blood routine,biochemistry and important organ structure and function,it has high safety,and can slightly reduce blood D-dimer and reduc

关 键 词：下肢深静脉血栓形成 活血中药透皮制剂 垫枕 犬 

分 类 号：R543.6[医药卫生—心血管疾病] R364.1[医药卫生—内科学]