华重楼不同部位的UPLC-Q-TOF-MS/MS定性分析和HPLC含量测定  

Qualitative and quantitative analysis of Paris polyphylla var. chinensis by UPLC-Q-TOF-MS/MS and HPLC

作　　者：李燕敏[1,2] 关亮俊 陈两绵 赵猛[1] 丁立帅 孟辰笑凝 高慧敏 王智民[1] LI Yan-min;GUAN Liang-jun;CHEN Liang-mian;ZHAO Meng;DING Li-shuai;MENG Chen-xiao-ning;GAO Hui-min;WANG Zhi-min(National Engineering Laboratory of Quality Control Technology of Chinese Materia Medica,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Beijing Health Vocational College,Beijing 100053,China)

机构地区：[1]中国中医科学院中药研究所中药质量控制技术国家工程实验室,北京100700 [2]北京卫生职业学院,北京100053

出　　处：《中国中药杂志》2021年第12期2900-2911,共12页China Journal of Chinese Materia Medica

基　　金：国家重点研发计划“中医药现代化研究”专项(2017YFC1701900);国家中药标准化项目(ZYBZH-Y-FJ-09);中医药行业科研专项(201507002);中央级公益性科研院所基本科研业务费专项(ZXKT20012)。

摘　　要：重楼来源于百合科重楼属多年生植物云南重楼或七叶一枝花的根茎,资源短缺促使重楼种植业快速发展,从而每年产生大量的非药用资源得不到有效利用而被废弃。为了明确华重楼(即七叶一枝花)根茎、须根、茎、叶、种子和果皮的化学组成,挖掘其非药用部位的综合利用价值和开发前景,该研究采用超高效液相串联四极杆飞行时间质谱(UPLC-QTOF-MS/MS)技术,结合HPLC对不同部位进行了定性、定量分析。从不同部位中共检测到136个化合物,其中112个甾体皂苷,6个黄酮,11个含氮化合物和7个植物甾醇;根茎、须根和种子的成分相似,均以偏诺皂苷为主;叶和茎以薯蓣皂苷类为主;果皮同时含有偏诺皂苷和薯蓣皂苷类,但以偏诺皂苷含量较高。从《中国药典》重楼含量测定指标看,华重楼不同部位4种皂苷总量以须根和根茎中最高,果皮及带假种皮的种子含量次之,叶中含量高于茎和无假种皮的种子。该研究为华重楼非药用部位的综合开发和合理利用提供了有效的数据支撑。Paridis Rhizoma(PR) is prepared from the dried rhizome of Paris polyphylla var. yunnanensis(PPY) or P. polyphylla var. chinensis(PPC) in Liliaceae family. The rapid development of PPY or PPC planting industry resulted from resource shortage has caused the waste of a large number of non-medicinal resources. To clarify the chemical compositions in rhizomes, fibrous roots, stems, leaves, seeds and pericarps of PPC, and explore the comprehensive application value and development prospect of these parts, the qualitative and quantitative analyses on the different parts of PPC were carried out by ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS) and high performance liquid chromatography(HPLC). A total of 136 compounds were identified, including 112 steroidal saponins, 6 flavonoids, 11 nitrogen-containing compounds and 7 phytosterols. Rhizomes, fibrous roots, and seeds mainly contained protopennogenyl glycosides and pennogenyl glycosides;leaves and stems mainly contained protodiosgenyl glycosides and diosgenyl glycosides;pericarps mainly contained pennogenyl glycosides, followed by diosgenyl glycosides. The total level of four saponins was the highest in fibrous roots and rhizomes, followed by those in the pericarps and arillate seeds, and the lowest in the stems and exarillate seeds. This study can provide data support for the comprehensive development and rational application of non-medicinal parts of PPC.

关 键 词：重楼 华重楼 非药用部位 化学组成 甾体皂苷 

分 类 号：R284.1[医药卫生—中药学]