瓜尤茶叶的三萜类成分研究  

Triterpenoids from leaves of Ilex guayusa

作　　者：黄惠[1,2] 陈烨 曾瑚瑚 黄晓君 王磊[1,2] 王英 马志国[1,2] 张晓琦 范春林[1,2,3] 叶文才 HUANG Hui;CHEN Ye;ZENG Hu-hu;HUANG Xiao-jun;WANG Lei;WANG Ying;MA Zhi-guo;ZHANG Xiao-qi;FAN Chun-lin;YE Wen-cai(Center for Bioactive Natural Molecules and Innovative Drugs Research,College of Pharmacy,Jinan University,Guangzhou 510632,China;Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research,Jinan University,Guangzhou 510632,China;NMPA Key Laboratory for Quality Evaluation of Traditional Chinese Medicine(Traditional Chinese Patent Medicine),Guangzhou 510632,China)

机构地区：[1]暨南大学天然生物活性分子和创新药物研究中心,广东广州510632 [2]广东省中药药效物质基础和创新药物研究重点实验室,广东广州510632 [3]国家药品监督管理局中成药质量评价重点实验室,广东广州510632

出　　处：《中国中药杂志》2021年第12期3123-3132,共10页China Journal of Chinese Materia Medica

基　　金：国家重点研发计划项目(2017YFC1703800);广东珠江人才计划本土创新科研团队项目(2017BT01Y036);国家自然科学基金项目(82074116)。

摘　　要：研究冬青科植物瓜尤茶Ilex guayusa叶的化学成分。采用硅胶、Sephadex LH-20和ODS柱色谱以及半制备型HPLC等方法从瓜尤茶干燥叶的95%乙醇提取物中分离得到16个三萜类化合物,通过核磁共振、高分辨质谱及文献比对等方法鉴定了这些化合物的结构,分别为3β-hydroxy-11α,12α-epoxy-24-nor-urs-4(23)-ene-28,13β-olide(1)、3β-hydroxy-24-nor-4(23),12-oleanadien-28-methyl ester(2)、齐墩果酸(3)、3β,28-dihydroxy-12-oleanene(4)、2α,3β-dihydroxy-11α,12α-epoxy-24-nor-olean-4(23)-ene-28,13β-olide(5)、熊果酸(6)、23-羟基熊果酸(7)、熊果醇(8)、3β-28-nor-urs-12-ene-3,17-diol(9)、3β-hydroxyurs-11-ene-28,13β-olide(10)、13β,28-epoxy-3β-hydroxy-11-ursene(11)、3β-hydroxy-28,28-dimethoxy-12-ursene(12)、3β-hydroxy-24-nor-urs-4(23),12-dien-28-oic acid(13)、3β-hydroxy-24-nor-urs-4(23),12-dien-28-methyl ester(14)、2α,3β-dihydroxy-11α,12α-epoxy-24-nor-urs-4(23)-ene-28,13β-olide(15)、2α,3β-dihydroxy-11α,12α-epoxy-24-nor-urs-4(23),20(30)-dien-28,13β-olide(16)。其中化合物12为新化合物,45、7、916为首次从瓜尤茶中分离得到。The chemical constituents from the leaves of Ilex guayusa were investigated.Sixteen triterpenoids were isolated from the 95%ethanol extract of dried leaves of I.guayusa by silica gel,Sephadex LH-20,and ODS column chromatographies and semi-prepa-rative HPLC.Those triterpenoids were identified by NMR,HR-MS,and literature analysis:3β-hydroxy-11α,12α-epoxy-24-nor-urs-4(23)-ene-28,13β-olide(1),3β-hydroxy-24-nor-4(23),12-oleanadien-28-methyl ester(2),oleanolic acid(3),3β,28-dihydroxy-12-oleanene(4),2α,3β-dihydroxy-11α,12α-epoxy-24-′nor-olean-4(23)-ene-28,13β-olide(5),ursolic acid(6),3β,23-dihydroxy ursolic acid(7),3β,28-dihydroxy-12-ursene(8),3β-28-nor-urs-12-ene-3,17-diol(9),3β-hydroxyurs-11-ene-28,13β-olide(10),13β,28-epoxy-3β-hydroxy-11-ursene(11),3β-hydroxy-28,28-dimethoxy-12-ursene(12),3β-hydroxy-24-nor-urs-4(23),12-dien-28-oic acid(13),3β-hydroxy-24-nor-urs-4(23),12-dien-28-methyl ester(14),2α,3β-dihydroxy-11α,12α-epoxy-24-nor-urs-4(23)-ene-28,13β-olide(15)and 2α,3β-dihydroxy-11α,12α-epoxy-24-nor-urs-4(23),20(30)-dien-28,13β-olide(16).Compounds 1-2 were new compounds,and compounds 4-5,7 and 9-16 were isolated from I.guayusa for the first time.

关 键 词：瓜尤茶 冬青科 化学成分 三萜 

分 类 号：R284.1[医药卫生—中药学]