miR-628-3p对胃癌细胞HGC-27增殖和迁移能力的影响  

Study on the effect of miR-628-3p on the proliferation and migration ability of gastric cancer cell HGC-27

作　　者：周超毅 周少波[1] 褚亮[1] 许兆龙 蒋磊[1] 单二波[1] 夏泽亮[1] 王法宝 Zhou Chaoyi;Zhou Shaobo;Chu Liang;Xu Zhaolong;Jiang Lei;Shan Erbo;Xia Zeliang;Wang Fabao(The Second Affiliated Hospital of Benghu Medical College,Benghu 233000,China)

机构地区：[1]蚌埠医学院第二附属医院普外科,安徽蚌埠233000

出　　处：《山东第一医科大学（山东省医学科学院）学报》2021年第7期488-491,共4页Journal of Shandong First Medical University ＆ Shandong Academy of Medical Sciences

基　　金：蚌埠医学兜自然科学项目(BYKY2019136ZD,BYKY2019138ZD,BYKY2019153ZD)。

摘　　要：目的初步探究miR-628-3p对胃癌细胞系HGC-27增殖和迁移能力的影响。方法qRT-PCR检测人正常胃黏膜细胞GES-1及HGC-27细胞miR-628-3p的表达水平。转染miR-628-3p模拟物后MTT法检测HGC-27细胞增殖,Transwell小室实验检测HGC-27细胞迁移能力Western blot检测cyclinDl蛋白表达水平。结果与正常胃黏膜细胞相比,HGC-27细胞miR-628-3p表达显著降低(P<0.01);与转染阴性对照组细胞相比,转染miR-628-3p模拟物组细胞活力显著降低(P<0.01),转染miR-628-3p模拟物的HGC-27细胞穿过小室聚碳酸酯膜的细胞数目明显减少(P<0.01),细胞增殖蛋白cyclinDl表达显著下调(P<0.01)。结论miR-628-3p能够降低cyclinDl表达,抑制HGC-27细胞增殖和迁移。Objective:To preliminarily explore the effect of miR-628-3p on the proliferation and migration ability ofgastric cancer cell line(HGC-27).Methods:qRT-PCR was used to detect the expression level of miR-628-3p in humannormal gastric mucosal cells(CES-1)and HCC-27 cells.The MTT method was used to detect the effet of overexpression ofmiR-628-3p on the proliferation of HCC-27 cells,and the Transwell chamber test was used to detect the effect on themigration ability of HCC-27 cells.Western blot was used to deteet the expression level of eyelinD1 protein.Resuls:Compared with normal gastric mucosal cells the expression of miR-628-3p in HCC-27 cells was significantly reduced(P<0.01);Compared with the negative control cells,the cell viability of the miR-628-3p mimic translected group wassignificantly reduced(P<0.01),the number of HCC-27 cells transfected with miR-628-3p mimics through the cellpolycarbonate membrane was significantly reduced(P<0.01),and the expression of cell proliferation protein eyclinD1 wassignificantly down-regulated(P<0.01).Conclusion:miR-628-3p can reduce the expression of cyclinD1 and inhibit theproliferation and migration of HGC-27 cells.

关 键 词：miR-628-3p 胃癌 细胞增殖 迁移 

分 类 号：R34[医药卫生—基础医学]