甲型和乙型流感病毒感染患儿淋巴细胞亚群和IL-6的表达  

Expressions of lymphocyte subsets and IL-6 in children infected with influenza A and B viruses

作　　者：薛燕 许俐[1] 党利亨[1] 王朝 支秀芳 徐勇胜[2] XUE Yan;XU Li;DANG Liheng(Institute of Pediatrics,Tianjin Children’s Hospital,Tianjin 300134,CHINA)

机构地区：[1]天津市儿童医院(天津大学儿童医院)儿科研究所,300134 [2]天津市儿童医院(天津大学儿童医院)呼吸科,300134 [3]天津市儿童医院(天津大学儿童医院)天津市儿童出生缺陷防治重点实验室,300134 [4]天津医科大学研究生院

出　　处：《江苏医药》2021年第7期689-692,共4页Jiangsu Medical Journal

基　　金：天津市科技计划项目(20JCZXJC00170)。

摘　　要：目的探讨甲型和乙型流感病毒感染患儿淋巴细胞亚群和IL-6的表达。方法采用流式细胞术检测59例甲型流感患儿(甲流组)、56例乙型流感患儿(乙流组)和46例健康儿童(对照组)外周血淋巴细胞亚群比率和计数。采用ELISA法测定甲流组和乙流组血清IL-6水平。结果甲流组和乙流组淋巴细胞计数均低于对照组(P<0.05)。与对照组相比,甲流组和乙流组CD3^(+)CD8^(+)淋巴细胞比率降低,CD4^(+)/CD8^(+)比值升高(P<0.05),CD3^(+)、CD3^(+)CD8^(+)淋巴细胞计数减少(P<0.01)。甲流组CD3^(+)淋巴细胞比率和CD3^(-)CD16^(+)/CD56^(+)淋巴细胞计数低于对照组(P<0.05)。乙流组CD3^(-)CD19^(+)淋巴细胞计数低于对照组(P<0.05)。甲流组和乙流组血清IL-6水平分别为15.31(6.20,38.81) pg/mL和11.04(6.26,30.08) pg/mL,比较无统计学差异(P>0.05)。结论甲型和乙型流感病毒感染患儿细胞免疫功能降低,有较多患儿出现一定程度的炎症反应。Objective To investigate the expressions of lymphocyte subsets and IL-6 in the children infected with influenza A and B viruses.Methods The ratio and count of lymphocyte subsets were detected in 59 children infected with influenza A virus(group A),56 children infected with influenza B virus(group B) and 46 healthy children(group C) by flow cytometry.Serum level of IL-6 was detected in group A and group B by ELISA.Results The lymphocyte count was lower in groups of A and B than that in group C(P<0.05).Compared to group C,the ratio of CD3^(+)CD8^(+) lymphocyte and the counts of CD3^(+) and CD3^(+)CD8^(+) lymphocyte were lower and the ratio of CD4^(+)/CD8^(+) was higher in groups of A and B(P<0.05).The ratio of CD3^(+) lymphocyte and the count of CD3^(-)CD16^(+)/CD56^(+) lymphocyte were lower in group A than those in group C(P<0.05).The count of CD3^(-)CD19^(+) lymphocyte was lower in group B than that in group C(P<0.05).Serum levels of IL-6 in groups of A and B were 15.31(6.20,38.81) pg/mL and 11.04(6.26,30.08) pg/mL,respectively, which was not significantly different between the two groups(P>0.05).Conclusion The cellular immune function of the children infected with influenza A and B viruses is reduced and more children have a certain degree of inflammatory response.

关 键 词：甲型流感 乙型流感 淋巴细胞亚群 白细胞介素6 儿童 

分 类 号：R725[医药卫生—儿科]