大叶井口边草乙酸乙酯部位化学成分研究  

Chemical Constituents from Ethyl Acetate Extract of Pteris cretica var.nervosa

作　　者：吴飞 万仲贤 徐元翠 张思波 郑良栋 刘磐 WU Fei;WAN Zhong-xian;XU Yuan-cui;ZHANG Si-bo;ZHENG Liang-dong;LIU Pan(The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture,Enshi 445000,China;Dongguan HEC Pharm R&D Co.Ltd.,Dongguan 523000,China)

机构地区：[1]恩施土家族苗族自治州中心医院,湖北恩施445000 [2]东莞东阳光药物研发有限公司,广东东莞523000

出　　处：《中药材》2020年第6期1368-1372,共5页Journal of Chinese Medicinal Materials

基　　金：湖北省自然科学基金项目(2019CFB615)。

摘　　要：目的:研究大叶井口边草乙酸乙酯部位的化学成分。方法:采用硅胶柱色谱、Sephadex LH-20柱色谱、RP-C18柱色谱和半制备高效液相色谱法对大叶井口边草乙酸乙酯部位的化学成分进行分离纯化,通过理化性质、1H-NMR、13C-NMR和MS等方法鉴定化合物结构。结果:从大叶井口边草乙酸乙酯部位中分离纯化了17个化合物,分别鉴定为:ent-3β-hydroxy-kaur-16-ene(1)、ent-2β,15α-dihydroxy-kaur-16-ene(2)、ent-3β,19-dihydroxy-kaur-16-ene(3)、ent-2β,6β,15α-trihydroxy-kaur-16-ene(4)、蕨素B(5)、芹菜素(6)、木犀草素(7)、槲皮素(8)、6-甲氧基槲皮素(9)、6-甲氧基柚皮素(10)、槲皮素-3-O-β-D-吡喃葡萄糖苷(11)、木犀草苷(12)、芹菜素-7-O-β-D-葡萄糖苷(13)、芹菜素-7-O-β-D-葡萄糖醛酸苷甲酯(14)、咖啡酸(15)、咖啡酸甲酯(16)、β-谷甾醇(17)。结论:其中,化合物1、3、7~16为首次从该植物中分离得到。Objective: To study the chemical constituents from the ethyl acetate extract of Pteris cretica var.nervosa.Methods: The chemical constituents from the ethyl acetate extract of Pteris cretica var.nervosa were isolated and purified by silica gel column chromatography, Sephadex LH-20 column chromatography, RP-C18 column chromatography and semi-preparative HPLC.Their structures were identified by physicochemical properties, 1H-NMR,13C-NMR and MS.Results: Seventeen compounds were isolated from the ethyl acetate extract of Pteris cretica var.nervosa and identified as ent-3β-hydroxy-kaur-16-ene(1),ent-2β,15α-dihydroxy-kaur-16-ene(2),ent-3β,19-dihydroxy-kaur-16-ene(3),ent-2β,6β,15α-trihydroxy-kaur-16-ene(4),pterosin B(5),apigenin(6),luteolin(7),quercetin(8),6-methoxyquercetin(9),6-methoxynaringenin(10),quercetin-3-O-β-D-glucopyranoside(11),luteoloside(12),apigenin-7-O-β-D-glucoside(13),apigenin-7-O-β-D-glucuronide methyl ester(14),caffeic acid(15),caffeic acid methyl ester(16),β-sitosterol(17),respectively.Conclusion: Among them, compounds 1,3,7 to 16 are isolated from Pteris cretica var.nervosa for the first time.

关 键 词：凤尾蕨属 大叶井口边草 化学成分 二萜 黄酮 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]