盐酸小檗碱复合纳米乳对高脂血症大鼠的影响  

Effect of berberine hydrochloride composite nanoemulsion on hyperlipidemia rats

作　　者：秦红 袁誉铭 杨婕 张景勍[1] 刘颖菊[1,3] QIN Hong;YUAN Yu-ming;YANG Jie;ZHANG Jing-qin;LIU Ying-ju(Chongqing Research Center for Pharmaceutical Engineering,College of Pharmacy,Chongqing Medical University,Chongqing 400016,China;Scientific Research Department,Children’s Hospital of Chongqing Medical University,Chongqing 400014,China;Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology,Chongqing 400016,China)

机构地区：[1]重庆医科大学药学院重庆药物高校工程研究中心,重庆400016 [2]重庆医科大学附属儿童医院科研处,重庆400014 [3]重庆市生物化学与分子药理学重点实验室,重庆400016

出　　处：《中国临床药理学杂志》2021年第15期2019-2022,共4页The Chinese Journal of Clinical Pharmacology

基　　金：重庆市社会事业与民生保障科技创新专项基金资助项目(cstc2017shmsA130028)。

摘　　要：目的研究盐酸小檗碱复合纳米乳(BHNE)对高脂血症大鼠的降脂作用。方法按照体重将SD大鼠随机分为7组:正常组(0.5%羧甲基纤维素钠),模型组(0.5%羧甲基纤维素钠),辛伐他汀组(辛伐他汀2 mg·kg^(-1)),低、高2个剂量盐酸小檗碱组(BEH-L,40,80 mg·kg^(-1)),低、高2个剂量盐酸小檗碱复合纳米乳组(BHNE-L,40,80 mg·kg^(-1)),每组6只;给药体积为10 mL·kg^(-1),每日1次,连续给药10周。除正常组外,其余各组均口服高脂饲料以诱导大鼠高脂血症模型。通过全自动生化分析仪测定高脂血症大鼠血清指标总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C),用试剂盒测定血清超氧化物歧化酶(SOD)水平和丙二醛(MDA)含量。结果正常组、模型组、辛伐他汀组、BEH-L、BEH-H、BHNE-L和BHNE-H组的TC含量分别为(1.34±0.26),(2.00±0.13),(1.40±0.28),(1.65±0.34),(1.56±0.46),(1.67±0.29)和(1.48±0.28)mmol·L^(-1);这7组的TG含量分别为(0.87±0.15),(1.17±0.24),(0.80±0.15),(1.09±0.17),(0.90±0.24),(0.94±0.33)和(0.83±0.21)mmol·L^(-1);这7组的HDL-C含量分别为(1.44±0.20),(0.61±0.15),(0.99±0.14),(0.81±0.23),(0.83±0.18),(0.87±0.26)和(0.86±0.23)mmol·L^(-1);这7组的LDL-C含量分别为(0.39±0.10),(0.50±0.11),(0.41±0.14),(0.49±0.07),(0.47±0.30),(0.49±0.26)和(0.42±0.18)mmol·L^(-1);这7组的SOD水平分别为(183.19±19.00),(150.76±6.30),(161.34±5.64),(138.05±10.00),(154.55±13.10),(145.96±13.92)和(163.68±10.06)U·mL^(-1);这7组的MDA水平分别为(5.38±0.66),(9.87±0.64),(8.32±0.36),(9.90±0.71),(9.04±1.23),(9.76±0.40)和(8.46±0.74)nmol·mL^(-1)。BHNE和BEH干预均能降低血清TC、TG和LDL-C水平,升高血清HDL-C的水平。BHNE的作用较等剂量的游离BEH更强。结论BHNE可有效改善实验性高脂血症大鼠的脂质水平,且比游离的BEH效果更好。Objective To study the lipid-lowering effect of berberine hydrochloride composite nanoemulsion(BHNE)on experimental hyperlipidemia rats.Methods The SD rats were randomly divided into 7 groups according to body weight:normal group(0.5%sodium carboxymethyl cellulose),model group(0.5%sodium carboxymethyl cellulose),simvastatin group(2 mg·kg^(-1)simvastatin),berberine hydrochloride(BEH)low and high dose groups(40,80 mg·kg^(-1)BEH),BHNE low and high-dose groups(40,80 mg·kg^(-1)BHNE),with 6 rats in each group;all rats ere given intragastric administration ith a volume of 10 m L·kg^(-1),once a day,for 10 weeks.Except for the normal group,the others were fed with high fat diet for established the hyperlipidemia model.The serum indexes cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-C)and low density lipoprotein cholesterol(LDL-C)of hyperlipidemia rats were detected by automatic biochemical analyzer.he serum superoxide dismutase(SOD)and malonaldehyde(MDA)content were detected with the kit.Results The levels of TC in normal group,model group,simvastatin group,BEH-L,BEH-H,BHNE-L and BHNE-H groups were(1.34±0.26),(2.00±0.13),(1.40±0.28),(1.65±0.34),(1.56±0.46),(1.67±0.29)and(1.48±0.28)mmol·L^(-1),respectively;the levels of TG in the seven groups were(0.87±0.15),(1.17±0.24),(0.80±0.15),(1.09±0.17),(0.90±0.24),(0.94±0.33),(0.83±0.21)mmol·L^(-1),respectively;the levels of HDL-C in the seven groups were(1.44±0.20),(0.61±0.15),(0.99±0.14),(0.81±0.23),(0.83±0.18),(0.87±0.26),(0.86±0.23)mmol·L^(-1),respectively;the levels of LDL-C in the seven groups were(0.39±0.10),(0.50±0.11),(0.41±0.14),(0.49±0.07),(0.47±0.30),(0.49±0.26),(0.42±0.18)mmol·L^(-1),respectively;the levels of SOD in the seven groups were(183.19±19.00),(150.76±6.30),(161.34±5.64),(138.05±10.00),(154.55±13.10),(145.96±13.92),(163.68±10.06)U·mL^(-1),respectively;the levels of MDA in the seven groups were(5.38±0.66),(9.87±0.64),(8.32±0.36),(9.90±0.71),(9.04±1.23),(9.76±0.40),(8.46±0.74)nmol·mL^

关 键 词：盐酸小檗碱 纳米乳 复合物 高脂血症 血脂水平 氧化应激 

分 类 号：R97[医药卫生—药品]