菟丝子拟雌激素作用体内直接作用物质的发现  

Direct acting substances discovery of estrogen effect of Cuscuta chinensis in vivo

作　　者：孙向明 宋辉[1] 赵丽珠 胡扬[1] 辛科颖 李文兰[1] 丁振铎 SUN Xiang-ming;SONG Hui;ZHAO Li-zhu;HU Yang;XIN Ke-ying;LI Wen-lan;DING Zhen-duo(College of Pharmacy,Harbin University of Commerce,Harbin 150076,China)

机构地区：[1]哈尔滨商业大学药学院,黑龙江哈尔滨150076

出　　处：《药学学报》2021年第7期1826-1831,共6页Acta Pharmaceutica Sinica

基　　金：黑龙江省普通本科高等学校青年创新人才培养计划项目(UNPYSCT-2018135);哈尔滨商业大学博士科研启动基金(2019DS115).

摘　　要：通过“代谢产物-效应检识”模式进行相关性分析,初步明确菟丝子在体内发挥拟雌激素作用的直接作用物质。以去势(卵巢摘除)雌性大鼠为研究对象,灌胃给予菟丝子的95%乙醇提取部位、大孔树脂40%乙醇洗脱部位、正丁醇萃取部位作为给药部位,采用UPLC/Q-TOF-MS技术建立不同给药部位、不同给药次数的血清指纹图谱。同时检测血清中雌二醇(E2)、促卵泡激素(FSH)、促黄体生成素(LH)水平,并进行双变量相关分析和灰关联度分析,筛选雌激素效应组分。结果表明,含药血清中发现9个与雌激素效应高度相关的体内直接作用物质,分别是:金丝桃苷、紫云英苷、甲基化槲皮素葡萄糖醛酸苷、槲皮素-二葡萄糖醛酸苷、槲皮素、芹菜素、异槲皮苷、山柰酚葡萄糖醛酸苷和山柰酚。基于血清谱效相关性的研究思路可初步发现菟丝子拟雌激素作用的体内直接作用物质。为菟丝子雌激素作用药效物质的揭示和质量标志物的确证提供了初步参考。本实验获得了哈尔滨商业大学伦理委员会的批准(批准号:HSDU2020-065)。The direct acting substances of Cuscuta chinensis in vivo were preliminarily identified through the correlation analysis of"metabolites-effect identification"model.The ovariectomized female rats were i.g administered with 95%ethanol extract part,40%ethanol elution part and n-butanol extract part of Cuscuta chinensis.The serum fingerprints of different parts and times of administration were established by UPLC/Q-TOF-MS.At the same time,serum estradiol(E2),follicle-stimulating hormone(FSH)and luteinizing hormone(LH)levels were detected.Bivariate correlation analysis and grey correlation analysis were used to screen estrogenic components.The results showed that nine direct acting substances in vivo highly related to estrogen effect were found in the drug containing serum,which were hyperoside,astragalin,methyl quercetin glucuronide,quercetin-diglucuronide,quercetin,apigenin,isoquercitrin,kaempferol glucuronide and kaempferol.We can preliminarily screen out the direct acting substance of estrogen effect of Cuscuta chinensis in vivo based on the research idea of serum spectrum effect correlation.It provides a reliable basis for revealing the estrogeneffective substances of Cuscuta chinensis and confirming the quality markers.This experiment was approved by Harbin University of Commerce Ethics Committees(Approval No.HSDU2020-065).

关 键 词：菟丝子 拟雌激素作用 谱效相关性 超高效液相色谱-四极杆飞行时间串联质谱 体内直接作用物质 

分 类 号：R285.5[医药卫生—中药学]