慢性乙型肝炎患者记忆性CD3^(+)CD8^(+)CD45RO^(+)T淋巴细胞IL-2/IL-15Rβ表达和抗病毒治疗相关性研究  

Expression of IL-2/IL-15Rβon memory CD3+CD8+CD45RO+T cells in chronic hepatitis B patients undergoing antiviral treatment and its significance

作　　者：韩文正 陈青 杨江华[3] 吴晓鑫 刘克洲[4] 李小宁[1] Han Wenzheng;Chen Qing;Yang Jianghua;Wu Xiaoxin;Liu Kezhou;Li Xiaoning(Clinical Laboratory,the First Affiliated Hospital of Wannan Medical College,Wuhu 241001,China;Department of Nuclear Medicine,the First Affiliated Hospital of Wannan Medical College,Wuhu 241001,China;Department of Infectious Diseases,the First Affiliated Hospital of Wannan Medical College,Wuhu 241001,China;Department of Infectious Diseases,State Key Laboratory for Infectious Diseases Diagnosis and Treatment,the First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China)

机构地区：[1]皖南医学院附属第一医院检验科,芜湖241001 [2]皖南医学院附属第一医院核医学科,芜湖241001 [3]皖南医学院附属第一医院感染科,芜湖241001 [4]浙江大学医学院附属第一医院感染科,感染性疾病诊治国家重点实验室,杭州310003

出　　处：《中华临床感染病杂志》2021年第2期107-115,共9页Chinese Journal of Clinical Infectious Diseases

基　　金：安徽省自然科学基金(1908085QH325)。

摘　　要：目的:探究慢性乙型肝炎患者外周血单个核细胞(PBMC)中记忆性CD3^(+)CD8^(+)CD45RO^(+)T淋巴细胞IL-2/IL-15受体β亚基(IL-2/IL-15Rβ)表达及其与细胞增殖和分泌功能相关性,以及慢性活动性乙型肝炎(CAHB)患者治疗前后乙型肝炎病毒(HBV)感染相关指标、丙氨酸转氨酶(ALT)和CD3^(+)CD8^(+)CD45RO^(+)T淋巴细胞IL-2/IL-15Rβ表达变化。方法:选取2019年3月至2020年12月皖南医学院附属第一医院感染科慢性乙型肝炎住院及门诊患者184例,根据纳入和排除标准,CAHB组纳入68例,无症状HBV携带组纳入47例。同时选取同时期30例健康体检者纳入健康对照组。CAHB组患者接受核苷(酸)类药物抗病毒治疗并随访(8例失访),分别比较剩余30例乙型肝炎e抗原(HBeAg)阳性和30例HBeAg阴性患者治疗前后HBV感染相关指标、ALT和CD3^(+)CD8^(+)CD45RO^(+)T淋巴细胞IL-2/IL-15Rβ表达变化。多组间正态分布计量资料比较采用单因素方差分析;两组间正态分布计量资料比较采用配对样本t检验;两组间非正态分布计量资料比较采用Mann-Whitney U秩和检验。采用Pearson相关系数评估CAHB患者PBMC CD3^(+)CD8^(+)CD45RO^(+)T淋巴细胞IL-2/IL-15Rβ表达比例和平均荧光强度(MFI)与CD3^(+)T淋巴细胞中CD8^(+)CD45RO^(+)T淋巴细胞比例相关性。P<0.05为差异有统计学意义。结果:CAHB组患者PBMC CD3^(+)T淋巴细胞中CD8^(+)CD45RO^(+)T淋巴细胞比例为(8.6±3.7)%,高于无症状HBV携带组[(5.7±2.5)%]和健康对照组[(5.5±1.5)%],差异均有统计学意义(P均<0.05);CAHB组PBMC CD3^(+)CD8^(+)CD45RO^(+)T淋巴细胞IL-2/IL-15Rβ表达比例为(6.8±4.7)%,高于无症状HBV携带组[(4.7±2.8)%]和健康对照组[(4.3±2.2)%],差异均有统计学意义(P均<0.05);CAHB组PBMC CD3^(+)CD8^(+)CD45RO^(+)T淋巴细胞IL-2/IL-15RβMFI为(243±168),高于无症状HBV携带组(160±91)和健康对照组(160±63),差异均有统计学意义(P均<0.05)。CAHB组PBMC CD3^(+)CD8^(+)CD45RO^(+)T淋巴细胞IL-2/IL-15Rβ表Objective To investigate the expression of IL-2/IL-15 receptorβsubunit(IL-2/IL-15Rβ)on memory CD3^(+)CD8^(+)CD45RO^(+)T cells in patients with chronic hepatitis B(CHB)receiving antiviral treatment and its significance.Methods Sixty-eight patients with chronic active hepatitis B(CAHB)and 47 asymptomatic hepatitis B virus(HBV)carriers attending in the Department of Infectious Diseases,the First Affiliated Hospital of Wannan Medical College from March 2019 to December 2020 were enrolled in the study;and 30 health subjects were also enrolled as healthy control group.Among 60 CAHB patients there were 30 cases with positive HBeAg and 30 cases with negative HBeAg.All CAHB patients received nucleos(t)ide analogue therapy,the HBV-related markers,Alanine aminotransferase(ALT)and the expression of IL-2/IL-15Rβon CD3^(+)CD8^(+)CD45RO^(+)T cells were determined and compared between HBeAg-positive and negative patients,before and after treatment.Normal distribution measurement data among 3 groups were compared with One-way ANOVA;normal distribution measurement data between 2 groups were compared with paired samples t test;non-normal distribution measurement data between the two groups were compared with Mann-Whitney U test;Pearson’s correlation coefficient was performed for correlation analysis.P<0.05 was considered statistically significant.Results The proportion of CD8^(+)CD45RO^(+)T cells on PBMC CD3^(+)T cells in CAHB group[(8.6±3.7)%]was higher than that of asymptomatic HBV carriers group[(5.7±2.5)%]and healthy control group[(5.5±1.5)%](all P<0.05).The expression percentage of IL-2/IL-15Rβon PBMC CD3^(+)CD8^(+)CD45RO^(+)T cells in CAHB group[(6.8±4.7)%]was higher than that of asymptomatic HBV carriers group[(4.7±2.8)%]and healthy control group[(4.3±2.2)%](all P<0.05).The MFI of IL-2/IL-15Rβon PBMC CD3^(+)CD8^(+)CD45RO^(+)T cells in CAHB group(243±168)was higher than those of asymptomatic HBV carriers group(160±91)and healthy control group[160±63](all P<0.05).The expression percentage and MFI of IL-2/IL-15R

关 键 词：慢性乙型肝炎 乙型肝炎病毒 记忆性CD3^(+)CD8^(+)CD45RO^(+)T淋巴细胞 IL-2/IL-15受体β亚基 流式细胞术 

分 类 号：R512.62[医药卫生—内科学]