基于网络药理学的肿节风治疗前列腺癌的作用机制探讨  

Discussion on the mechanism of Glabrous Sarcandra Herb in treating prostate cancer based on network pharmacology

作　　者：李晓 李灿楦 林晏廷 徐争光 陈洁[1] LI Xiao;LI Can-xuan;LIN Yan-ting;Xu Zheng-guang;CHEN Jie(Department of Urology,the First Affiliated Hospital of Jinan University,Guangzhou 510632,China)

机构地区：[1]暨南大学附属第一医院泌尿外科,广东广州510632

出　　处：《现代药物与临床》2021年第7期1354-1360,共7页Drugs & Clinic

基　　金：国家自然科学基金资助项目(81871155)。

摘　　要：目的利用网络药理学方法探讨肿节风治疗前列腺癌的可能作用机制。方法通过TCMSP、Uniprot、GeneCards在线数据库筛选肿节风活性化合物治疗前列腺癌的潜在靶点,利用Cytoscape软件构建前列腺癌-肿节风-靶点网络图,然后结合STRING数据库和Cytoscape软件构建蛋白质相互作用网络;最后利用R软件对靶点进行GO分类富集分析和KEGG通路富集分析,预测其作用机制,并构建靶点通路网络。结果从肿节风中筛选出活性成分10个,其与前列腺癌共同作用靶点170个,GO功能分析发现靶点主要与膜伐、对氧水平、泛素样蛋白连接酶结合等有关。KEGG通路分析发现肿节风治疗前列腺癌主要与糖尿病并发症中的AGE-RAGE信号通路、前列腺癌(PCa)、流体剪切应力(FSS)与动脉粥样硬化(AS)等通路密切相关。结论肿节风活性成分可能通过多靶点、多通路达到治疗及辅助治疗前列腺癌的目的。Objective To explore the possible mechanism of Glabrous Sarcandra Herb in treatment of prostate cancer by network pharmacology. Methods The potential targets of active compounds of Glabrous Sarcandra Herb in treatment of prostate cancer were screened by TCMSP, Uniprot and GeneCards online databases. Cytoscape software was used to construct a network map of prostate cancer tumor targets, and then STRING database and Cytoscape software were combined to construct a protein interaction network. R software was used to perform GO classification enrichment analysis and KEGG pathway enrichment analysis on the targets.mechanism of Glabrous Sarcandra Herb in treatment of prostate cancer were predicted and a target pathway network was constructed.Results Ten active ingredients were screened out from Glabrous Sarcandra Herb, 170 targets that interacted with prostate cancer. GO function analysis revealed that the targets were mainly related to membrane cutting, para-oxygen level, and ubiquitin-like protein ligase binding. KEGG pathway analysis found that the AGE-RAGE signaling pathway, prostate cancer(PCa), fluid shear stress(FSS) and atherosclerosis(AS) pathways in the complications of diabetes were mainly related to Glabrous Sarcandra Herb in treatment of prostate cancer. Conclusion The active ingredients of Glabrous Sarcandra Herb may achieve the purpose of treatment and adjuvant treatment of prostate cancer through multiple targets and multiple pathways.

关 键 词：肿节风 前列腺癌 网络药理学 信号通路 AGE-RAGE信号通路 

分 类 号：R285.5[医药卫生—中药学]