人鼻病毒1B感染致人肺支气管上皮细胞代谢组改变的研究  

Metabolomic alterations of human lung bronchial epithelial cells infected with human rhinovirus 1B

作　　者：苏萌[1] 李丹[1] 孙萍萍 王春阳 王蒋丽[3] 王宏[4] 章青[4] 杜娈英[1] 谢广成[1] Su Meng;Li Dan;Sun Pingping;Wang Chunyang;Wang Jiangli;Wang Hong;Zhang Qing;Du Luanying;Xie Guangcheng(Department of Pathogenic Biology/Laboratory for Pathogens Prevention and Control,Chengde Medical University,Chengde 067000,China;Clinical Medical College,Xi′an Medical University,Xi′an 710021,China;Department of Microbiology Laboratory,Chengde Center for Disease Control and Prevention,Chengde 067000,China;National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]承德医学院病原生物学教研室/病原防控研究室,067000 [2]西安医学院临床学院,710021 [3]承德市疾病预防控制中心微生物检验室,067000 [4]中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《中华微生物学和免疫学杂志》2021年第7期545-549,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金(81702008,81702010);河北省自然科学基金(H2018406024);河北省高校重点学科(冀教高[2013]4号);河北省科技厅"技术创新引导专项-科技工作会商"项目。

摘　　要：目的探讨人鼻病毒1B(human rhinovirus 1B,HRV1B)感染对人肺支气管上皮细胞BEAS-2B代谢组改变的影响。方法HRV1B感染BEAS-2B细胞6 h和12 h后,采用非靶向代谢组学技术研究BEAS-2B细胞代谢组的改变情况。结果HRV1B感染BEAS-2B细胞6 h和12 h分别有93个差异显著代谢产物(differentially significant metabolites,DSMs)(上调47个DSMs、下调46个DSMs)和88个DSMs(上调37个DSMs、下调51个DSMs);HRV1B感染动力学(HRV1B 6 h∶12 h)过程中共鉴定到30个DSMs(上调12个DSMs、下调18个DSMs)。3组中未知DSMs占据比例均较大。脂肪酸、脂质、氨基酸、核苷酸和糖类的比例与HRV1B的感染时间呈现正相关,邻苯二甲酸二异壬酯等为HRV1B感染BEAS-2B细胞后的共同DSMs。结论HRV1B感染致BEAS-2B细胞的脂肪酸、脂质、氨基酸、核苷酸和糖类代谢物发生明显改变。Objective To research the metabolomic alterations of human lung bronchial epithelial cells infected with human rhinovirus 1B(HRV1B).Methods Untargeted metabolomics was used to determine the metabolomic alterations in human lung bronchial epithelial cells(BEAS-2B)6 h,12 h and during the dynamic process(6 h∶12 h)after HRV1B infection.Results A total of 93 differentially significant metabolites(DSMs)(47 DSMs were up-regulated and 46 DSMs were down-regulated)and 88 DSMs(37 DSMs were up-regulated and 51 DSMs were down-regulated)at post infection of HRV1B in BEAS-2B at 6 h or 12 h,respectively.A total of 30 DSMs(12 DSMs were up-regulated and 18 DSMs were down-regulated)in a dynamic process(6 h∶12 h)after HRV1B infection.Unknown metabolites took up most proportions.The trends of fatty acid,lipid,amino acid,nucleotide and carbohydrate were increased along with the prolonging of HRV1B infection.DSMs such as Diisononyl phthalate was co-detected DSMs among three groups.Conclusions Metabolites such as fatty acid,lipid,amino acid,nucleotide and carbohydrate of BEAS-2B cells are changed induced by HRV1B infection.

关 键 词：人鼻病毒1B 人肺支气管上皮细胞 代谢组 

分 类 号：R373[医药卫生—病原生物学]