Identification and characterization of long noncoding RNAs and their association with acquisition of blood meal in Culex quinquefasciatus  

作　　者：Azali Azlan Mardani Abdul Halim Faisal Mohamad Ghows Azzam 

机构地区：[1]School of Biological Sciences,Universiti Sains Malaysia,Penang,Malaysia

出　　处：《Insect Science》2021年第4期917-928,共12页昆虫科学（英文版）

基　　金：This study was funded by the Universiti Sains Malaysia Research University Grant(1001/PBIOLOGI/8011604);MOSTI Science-Fund(305/PBIOLOGI/613238).

摘　　要：The Southern house mosquito,Culex quinquefasciatus(Cx.quinquefasciatus)is an important vector that transmit multiple diseases including West Nile encephalitis,Japanese encephalitis,St.Louis encephalitis and lymphatic filariasis.Long noncoding RNAs(lncRNAs)involve in many biological processes such as development,infection,and virus–host interaction.However,there is no systematic identification and characterization of lncRNAs in Cx.quinquefasciatus.Here,we report the first lncRNA identification in Cx.quinquefasciatus.By using 31 public RNA-seq datasets,a total of 4763 novel lncRNA transcripts were identified,of which 3591,569,and 603 were intergenic,intronic,and antisense respectively.Examination of genomic features revealed that Cx.quinquefasciatus shared similar characteristics with other species such as short in length,low GC content,low sequence conservation,and low coding potential.Furthermore,compared to protein-coding genes,Cx.quinquefasciatus lncRNAs had lower expression values,and tended to be expressed in temporally specific fashion.In addition,weighted correlation network and functional annotation analyses showed that lncRNAs may have roles in blood meal acquisition of adult female Cx.quinquefasciatus mosquitoes.This study presents the first systematic identification and analysis of Cx.quinquefasciatus lncRNAs and their association with blood feeding.Results generated from this study will facilitate future investigation on the function of Cx.quinquefasciatus lncRNAs.

关 键 词：Culex quinquefasciatus BLOOD-FEEDING long noncoding RNA TRANSCRIPTOME 

分 类 号：Q96[生物学—昆虫学]