The microRNA miR-184 regulates the CYP303A1 transcript level to control molting of Locusta migratoria  

作　　者：Yan-Li Wang Li-Xian Wu Hui-Yong Li Xue-Qin Wen En-Bo Ma Kun-Yan Zhu Jian-Zhen Zhang 

机构地区：[1]Research Institute of Applied Biology,Shanxi University,Taiyuan,Shanxi,China [2]College of Life Science,Shanxi University,Taiyuan,Shanxi,China [3]Department of Entomology,Kansas State University,Manhattan,KS,USA

出　　处：《Insect Science》2021年第4期941-951,共11页昆虫科学（英文版）

基　　金：This work was supported by National Natural Science Foundation of China(Grant No.31730074,31761133021,31802018,31320103921);the Program for Top Young Academic Leaders of Higher Learning Institutions of Shanxi Province(TYAL)2017,Key Research and Development Program of Shanxi Province(201803D221004-5);The Natural Science Foundation of Shanxi Province,China(201801D221247).

摘　　要：Cytochrome P450 monooxygenases(CYPs)play essential physiological functions in insects.CYP303A1 is highly conserved in insect species studied to date,and shows an indispensable role for adult eclosion in both Locusta migratoria and Drosophila melanogaster.However,how CYP303A1 is regulated to control insect developmental processes remains uninvestigated.In this study,we discovered functional binding sites for miR-184 in the coding sequence of LmCYP303A1.The luciferase reporter assay showed that miR-184 could target LmCYP303A1 and regulate its expression in vitro.Furthermore,overexpression of miR-184 through microinjection of agomir to locusts reduced the transcripts of LmCYP303A1 and led to abnormal molting,which is similar to the phenotype of silencing LmCYP303A1 by direct injection of dsLmCYP303A1 to locusts.Meanwhile,down-regulation of miR-184 by injection of antagomir increased the LmCYP303A1 transcript and caused molting defects.These findings suggested that miR-184 could target LmCYP303A1 to regulate the molting process in L.migratoria,which might be considered as a novel target for pest control.

关 键 词：CYP303A1 gene regulation insect molting Locusta migratoria miR-184 

分 类 号：Q96[生物学—昆虫学]