Characterization and target gene analysis of microRNAs in the antennae of the parasitoid wasp Microplitis mediator  

作　　者：Shuang Shan Shan-Ning Wang Xuan Song Adel Khashaveh Zi-Yun Lu Khalid Hussain Dhiloo Rui-Jun Li Xi-Wu Gao Yong-Jun Zhang 

机构地区：[1]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing,China [2]College of Plant Protection,China Agricultural University,Beijing,China [3]Institute of Plant and Environment Protection,Beijing Academy of Agricultural and Forestry Sciences,Beijing,China [4]College of Plant Protection,Agricultural University of Hebei,Baoding,Hebei,China [5]IPM Center of Hebei Province,Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China,Ministry of Agriculture,Plant Protection Institute,Hebei Academy of Agricultural and Forestry Sciences,Baoding,Hebei,China [6]Department of Entomology,Faculty of Crop Protection,Sindh Agriculture University,Tandojam,Pakistan

出　　处：《Insect Science》2021年第4期1033-1048,共16页昆虫科学（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(31672038,31621064 and 31772176);the National Key Research and Development Program of China(2019YFD0300105 and 2017YFD0201900).

摘　　要：MicroRNAs(miRNAs),a class of non-coding single-strand RNA molecules encoded by endogenous genes,are about 21–24 nucleotides long and are involved in the post-transcriptional regulation of gene expression in plants and animals.Generally,the types and quantities of miRNAs in the different tissues of an organism are diverse,and these divergences may be related to their specific functions.Here we have identified 296 known miRNAs and 46 novel miRNAs in the antennae of the parasitoid wasp Microplitis mediator by high-throughput sequencing.Thirty-three miRNAs were predicted to target olfactory-associated genes,including odorant binding proteins(OBPs),chemosensory proteins,odorant receptors(ORs),ionotropic receptors(IRs)and gustatory receptors.Among these,17 miRNAs were significantly highly expressed in the antennae,four miRNAs were highly expressed both in the antennae and head or wings,while the remaining 12 miRNAs were mainly expressed in the head,thorax,abdomen,legs and wings.Notably,miR-9a-5p and miR-2525-3p were highly expressed in male antennae,whereas miR-1000-5p and novel-miR-13 were enriched in female antennae.The 17 miRNAs highly expressed in antennae are likely to be associated with olfaction,and were predicted to target one OBP(targeted by miR-3751-3p),one IR(targeted by miR-7-5p)and 14 ORs(targeted by 15 miRNAs including miR-6-3p,miR-9a-5p,miR-9b-5p,miR-29-5p,miR-71-5p,miR-275-3p,miR-1000-5p,miR-1000-3p,miR-2525-3p,miR-6012-3p,miR-9719-3p,novel-miR-10,novel-miR-13,novel-miR-14 and novel-miR-28).These candidate olfactory-associated miRNAs are all likely to be involved in chemoreception through the regulation of chemosensory gene expression in the antennae of M.mediator.

关 键 词：expression profile MIRNA Microplitis mediator olfactory-associated gene target gene 

分 类 号：Q96[生物学—昆虫学]