Performance of Bt-susceptible and -heterozygous dual-gene resistant genotypes of Spodoptera frugiperda (J.E.Smith) (Lepidoptera:Noctuidae) in seed blends of non-Bt and pyramided Bt maize  

作　　者：Marcelo Dimase Sebe Brown Graham P.Head Paula A.Price Wade Walker Wenbo Yu Fangneng Huang 

机构地区：[1]Department of Entomology,Louisiana State University Agricultural Center,Baton Rouge,Louisiana,USA [2]Dean Lee Research Station,Louisiana State University Agricultural Center,Alexandria,Louisiana,USA [3]Bayer Crop Science,Chesterfield,Missouri,USA [4]Macon Ridge Research Station,Louisiana State University Agricultural Center,Winnsboro,Louisiana,USA

出　　处：《Insect Science》2021年第4期1147-1158,共12页昆虫科学（英文版）

基　　金：This article is published with the approval of the Director of the Louisiana Agri-cultural Experiment Station as manuscript No.2020-234-34595;This project represents work supported by Bayer Crop Science(St.Louis,MO,USA),USDA Regional Research Project NC-246;Hatch funds from the USDA National Institute of Food and Agriculture.

摘　　要：A seed blend refuge has been implemented in the U.S.Corn Belt for Bt maize resistance management.The fall armyworm,Spodoptera frugiperda(J.E.Smith),is a target pest of Bt maize in the Americas.The larvae of this pest are mobile,which may affect the efficacy of seed blend refuges.In this study,field and greenhouse trials were conducted to determine the performance of Bt-susceptible(aabb)and-heterozygous dual-gene-resistant(AaBb)genotypes of S.frugiperda in seed blends of non-Bt and pyramided Bt maize.Three field trials evaluated larval survival,larval growth,and plant injury with aabb in seed blends of Bt maize expressing Cry1A.105/Cry2Ab2/Vip3A with 0–30%non-Bt seeds.Greenhouse tests investigated the performance of aabb and AaBb in seed blends of Cry1A.105/Cry2Ab2 with 0–30% non-Bt seeds.In pure non-Bt maize plots,after 9–13 d of neonates being released on the plants,0.39 and 0.65 larvae/plant survived with leaf injury ratings of 4.7 and 5.9(Davis's 1–9 scale)in the field and greenhouse,respectively.In contrast,live larvae and plant injury were virtually not observed on Bt plants across all planting patterns.Larval occurrence and plant injury by aabb on non-Bt plants were similar between seed blends and pure non-Bt plantings,suggesting that the blended refuges could provide an equivalent susceptible population as structured refuge under the test conditions.In the greenhouse,the two insect genotypes in seed blends performed similarly,indicating that the seed blends did not provide more favorable conditions for AaBb over aabb.The information generated from this study should be useful in managing S.frugiperda and evaluating if send blends could be suitable refuge options for Bt resistance management in the regions where the insect is a primary target pest.

关 键 词：Bt maize dual-gene resistance fall armyworm resistance management seed blend 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]