HPLC法测定百合固金片中甘草酸的含量  

Determination of Glycyrrhizic Acid in BaiHe GuJin Tablets by HPLC

作　　者：冯娟[1] 张晓明 覃亮 FENG Juan;ZHANG Xiao-ming;QIN Liang(GuangDong Foo d and Drug Vocational College,Guangzhou 510520,China;Zhaoqing University,Zhaoqing 526061,China)

机构地区：[1]广东食品药品职业学院,广东广州510520 [2]肇庆学院食品与制药工程学院,广东肇庆526061

出　　处：《海峡药学》2021年第7期54-56,共3页Strait Pharmaceutical Journal

基　　金：2017年广东省本科高校教学质量与教学改革项目-重点专业建设项目,NO.359。

摘　　要：目的建立一种基于HPLC的测定百合固金片中甘草酸含量的方法。方法以JA DE-PAK C 18(5μm,250×4.6 mm)色谱柱为固定相,以40%乙腈(含有0.2%磷酸)为流动相,在流速1.0 mL·min^(-1),色谱柱温度30℃,波长252 nm的条件下测定百合固金片中甘草酸的含量。结果该方法专属性强,精密度高,稳定性、重复性好。结论该方法可以对百合固金片的质量进行有效的控制。OBJECTIVE To establish a method for determination of glycyrrhizic acid in BaiHe GuJin Tablets based on HPLC.METHODS A JADE-PAK C18(5μm,250×4.6 mm)column was used as the stationary phase,and 40%acetonitrile(containing 0.2%phosphoric acid)was used as the mobile phase.The content of glycyrrhizic acid in BaiHe GuJin tablets was determined under the conditions of flow rate 1.0 mL·min^(-1),column temperature 30℃and wavelength 252 nm.RESULTS The method had strong specificity,high precision,good stability and repeatability.CONCLUSION This method can effectively control the quality of BaiHe GuJin Tablets.

关 键 词：HPLC 百合固金片 甘草酸 含量测定 

分 类 号：R927[医药卫生—药学]