雪白睡莲花粗提物对奶牛乳房炎主要致病菌体外抑菌和抗炎作用  

Antimicrobial and Anti-inflammatory Effects of Crude Extract of Nymphaea candida on Major Pathogens in Cow Mastitis in Vitro

作　　者：刘丹丹 罗洁 向薇薇 况玲[1] 赛福丁·阿不拉[1] 侯宇[1] 买占海 张伟[1] LIU Dan-dan;LUO Jie;XIANG Wei-wei;KUANG Ling;SAI FUDING A-bula;HOU Yu;MAI Zhan-hai;ZHANG Wei(College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi,Xinjiang,830052,China)

机构地区：[1]新疆农业大学动物医学学院,新疆乌鲁木齐830052

出　　处：《动物医学进展》2021年第7期17-21,共5页Progress In Veterinary Medicine

基　　金：新疆维吾尔自治区自然科学基金项目(2018D01B19);2018年南农-新农联合基金项目(H)(18NN-XNLHJJ);新疆农业大学校前期项目(XJAU201716);新疆维吾尔自治区研究生科研创新项目(XJ2020G159);新疆农业大学校级科研创新项目(XJAUGRI2019028);新疆维吾尔自治区天山创新团队项目(2020D14005)。

摘　　要：为探索雪白睡莲花粗提物的体外抑菌和抗炎效果,用雪白睡莲花水提物、醇提物进行了体外抑菌试验,通过牛津杯法和倍比稀释法进行抑菌圈、最低抑菌浓度(MIC)及最低杀菌浓度(MBC)的测定,确定雪白睡莲花粗提物对奶牛乳房炎主要致病菌大肠埃希氏菌、金黄色葡萄球菌及链球菌的抑菌活性,并采用二甲苯致小鼠耳廓肿胀法观察其抗炎作用。结果显示,雪白睡莲花水提物和醇提物对金黄色葡萄球菌抑菌效果最好,且水提物抑菌效果优于醇提物;醇提物对金黄色葡萄球菌抑菌效果优于对链球菌的,对链球菌抑菌效果优于对大肠埃希氏菌的。与空白对照组相比,雪白睡莲花水提物、醇提物能明显抑制二甲苯所致小鼠耳廓肿胀(P<0.01),雪白睡莲花醇提物抑制率优于水提物抑制率。研究结果为雪白睡莲花用于治疗感染相关疾病提供理论依据。In order to explore the in vitro antibacterial and anti-inflammatory effects of the crude extract of Nymphaea candida,the water extract and the alcohol extract of Nymphaea candida were used for in vitro antibacterial test.The antibacterial circle,minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)were determined by Oxford cup method and multiple dilution method,and the main pathogenic bacteria of dairy cow mastitis were determined.The antibacterial activity of Staphylococcus aureus,Escherichia coli and Streptococcus agalactiae,and the anti-inflammatory effect of xylene induced auricle swelling in mice were observed.The results showed that the water extract and alcohol extract had the best antibacterial effect on Staphylococcus aureus,and the antibacterial effect of water extract was better than that of alcohol extract;the inhibitory effect of alcohol extract on Staphylococcus aureus was better than that on Streptococcus agalactiae,and the antibacterial effect on Streptococcus agalactiae was better than that on Escherichia coli.Compared with the blank control group,the water extract and alcohol extract of Nymphaea candida significantly inhibited xylene induced ear swelling in mice(P<0.01),and the inhibition rate of alcohol extract was better than that of water extract.The research results provided experimental basis for the development of new natural drugs for the treatment of infection-related diseases.

关 键 词：雪白睡莲花 奶牛乳房炎 体外抑菌 抗炎 

分 类 号：S857.26[农业科学—临床兽医学] S853.72[农业科学—兽医学]