骨髓间充质干细胞诱导调节性T细胞缓解心肌缺血-再灌注损伤  

Bone marrow mesenchymal stem cells protect myocardial ischemia-reperfusion injury by inducing regulatory T cells in mice

作　　者：庞凌霄[1] 李茜[1] 朱蔚[1] 盛斌[1] 袁永生[1] 欧英炜 安荣成 蔡文伟 Pang Lingxiao;Li Qian;Zhu Wei;Sheng Bin;Yuan Yongsheng;Ou Yingwei;An Rongcheng;Cai Wenwei(Department of Emergency Medicine,Zhejiang Provincial People's Hospital,the People's Hospital Affi liated to Hangzhou Medical College,Hangzhou 310014,China;不详)

机构地区：[1]浙江省人民医院,杭州医学院附属人民医院急诊医学科,杭州310014 [2]不详

出　　处：《中华急诊医学杂志》2021年第8期973-978,共6页Chinese Journal of Emergency Medicine

基　　金：浙江省医药卫生科技计划面上项目(2019KY018,2020KY438)。

摘　　要：目的探讨骨髓间充质干细胞(BM-MSC)对小鼠心肌缺血-再灌注损伤(MIRI)的保护作用及机制。方法24只C57小鼠随机(随机数字法)分为假手术组(sham operation SO组)、MIRI模型组(RI组)、MIRI模型MSC治疗组(MSC+RI组)、MIRI模型MSC治疗合并调节性T细胞(regulatory T cell,Treg)清除组(MSC+RI+PC61组)。流式细胞仪检测各组小鼠心脏中Treg的比例,ELISA法测血清中肌酸激酶(CK)、肌钙蛋白(TNI)、B型钠尿肽(BNP)、白介素10(interleukin 10,IL-10)和转化生长因子β(transforming Growth Factor,TGF-β)的含量,HE染色观察小鼠心肌组织学改变,TUNEL染色测定心肌细胞凋亡指数,TTC染色测定心肌梗死面积比。采用单因素方差分析统计数据。结果MSC+RI组较其余实验组相比小鼠心脏中Treg的比例、血清IL-10和TGF-β的数量最高,CK、TNI、BNP值最低(P<0.01),并且该组的心肌炎症细胞浸润,心肌凋亡指数和心肌梗死面积比,组织纤维化均最少(P<0.01)。结论MSC通过诱导Treg的产生,增加抑炎型细胞因子IL-10、TGF-β的释放,减轻心肌缺血-再灌注后的炎症损伤。Objective To investigate the protective effect and mechanism of bone marrowderived mesenchymal stem cell(BM-MSC)on myocardial ischemia-reperfusion injury(MIRI)in mice.Methods Twenty four C57 MIRI mice were randomly(random number)divided into four groups:SO group,RI group,MSC+RI group,and MSC+RI+PC61 group.The ratio of Treg were detected by fl ow cytometry.Serum levels of CK,TNI,BNP,IL-10 and TGF-β were measured by ELISA.The histological changes of myocardium were observed by HE staining.The number of cardiomyocyte apoptosis was measured by TUNEL staining,and the area ratio of myocardial infarction were determined by TTC staining.One-way ANOVA was used to analyze the data.Results In the MSC+RI group,the ratio of Treg and the levels of IL-10 and TGF-β were the highest,while CK,TNI and BNP were the lowest(P<0.01).The number of myocardial apoptotic cells,infarct size and tissue fi brosis were the least(P<0.01).Conclusions MSC can induce the production of Treg,increase the release of anti-infl ammatory cytokines IL-10 and TGF-β,and reduce the infl ammatory injury after myocardial ischemia-reperfusion.

关 键 词：骨髓间充质干细胞 调节性T细胞 缺血-再灌注损伤 炎症因子 

分 类 号：R542.22[医药卫生—心血管疾病]