益心通脉颗粒基于PI3K/AKT通路对CHD大鼠心肌损伤及应激水平的研究  

The study of Yixin Tongmai Granules on myocardial injury and stress level of rats with coronary heart disease based on PI3K/AKT pathway

作　　者：李楠[1] 洪林巍[1] 丛敬[1] 高嵋[1] 张桐桐 LI Nan;HONG Linwei;CONG Jing;GAO Mei;ZHANG Tongtong(Department of cardiology,the Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang Liaoning 110000,China)

机构地区：[1]辽宁中医药大学附属医院心血管内科,辽宁沈阳110000

出　　处：《转化医学杂志》2021年第4期212-217,共6页Translational Medicine Journal

基　　金：国家自然科学基金项目(81605304)。

摘　　要：目的探讨益心通脉颗粒基于PI3K/AKT通路对冠状动脉粥样硬化性心脏病(coronary atherosclerotic heart disease,CHD)大鼠心肌损伤及应激水平的研究。方法将60只PF级Wistar健康大鼠按照随机数字法分为6组:A组(正常组)、B组(CHD组)、C组(格列吡嗪药物对照组)、D组(低剂量益心通脉颗粒组)、E组(中剂量益心通脉颗粒组)及F组(高剂量益心通脉颗粒组),每组10只,除A组外其余均建立CHD动物模型,建模成功后,C组大鼠给予0.5 mg/kg格列吡嗪溶于2 ml蒸馏水灌胃;D组、E组及F组大鼠均给予1.25 mg/kg、2.5 mg/kg及5 mg/kg的益心通脉颗粒溶于2 ml蒸馏水灌胃;其余组别大鼠灌胃2 ml生理盐水。试剂盒采用血清氧化应激指标;HE染色观察心肌组织形态及结构;TUNEL法检测心肌细胞凋亡率;免疫组化及免疫印迹分别检测心肌组织中PI3K/AKT阳性率及蛋白表达。结果与A组相比,B组大鼠血清丙二醛(malondialdehyde,MDA)含量升高,NO、超氧化物歧化酶(super oxide dismutase,SOD)及谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)含量降低(P<0.05),与B组相比,C组、D组、E组、F组大鼠血清MDA降低,其余指标升高(P<0.05);A组心肌组织结构清晰,纤维布局完整,B组大鼠心肌组织分布紊乱及纤维溶解坏死,C组、D组、E组及F组大鼠心肌结构改善;与A组相比,B组大鼠心肌细胞凋亡率升高(P<0.05),C组、D组、E组及F组大鼠心肌细胞凋亡率均低于B组(P<0.05);B组大鼠心肌组织PI3K、AKT细胞阳性数量及蛋白水平均低于A组(P<0.05),与B组相比,C组、D组、E组及F组大鼠心肌组织PI3K、AKT细胞阳性数量及蛋白含量均升高(P<0.05),且呈现浓度依赖性F组大鼠氧化应激水平、心肌细胞凋亡及PI3K、AKT细胞阳性数量均优于D组及E组,与C组相比无明显意义(P<0.05)。结论益心通脉颗粒能够通过降低CHD大鼠氧化应激水平,减少心肌组织病理损伤及心肌细胞凋亡,这可能与上调PI3K/AKT通路表达相�Objective To investigate the effects of Yixin Tongmai granules on myocardial injury and stress level of rats with coronary heart disease based on the PI3K/AKT pathway.Methods 60 PF-class Wistar healthy rats were randomly divided into 6 groups including Group A(normal group),group B(CHD group),group C(glipizide drug control group),group D(low-dose Yixin Tongmai granule group),group E(medium-dose Yixin Tongmai granule group)and group F(high-dose Yixin Tongmai granule group),10 rats in each group.Except for group A,all others had established coronary heart disease.After the model was successfully built,Rats in group C were given 0.5 mg/kg glipizide dissolved in 2 ml distilled water by gavage.Rats in group D,group E,and group F were given 1.25 mg/kg,2.5 mg/kg and 5 mg/kg Yixintongmai granules dissolved in 2 ml distilled water for intragastric administration.The rats in the other groups were given 2 ml of normal saline by gavage.Results Compared to group A,the serum MDA content of group B was increased,while the contents of NO,SOD and GSH-Px were decreased(P<0.05).Compared to group B,serum MDA in groups C,D,E and F was decreased,while other indexes were increased(P<0.05).The myocardial tissue structure of group A was clear and the fiber layout was complete.The myocardial tissue distribution of group B was disordered and the fiber lytic necrosis was observed.The myocardial structure of groups C,D,E,and F was improved.Compared to group A,the apoptosis rate of myocardial cells in group B was increased(P<0.05).The apoptosis rate of myocardial cells in groups C,D,E and F was lower than that of in group B(P<0.05).The number of positively expressed cell and protein levels of PI3K and AKT in the myocardium of rats in group B were lower than those in group A(P<0.05).Compared with group B,the number of the positively expressed cell of PI3K and AKT and protein content in the myocardium of rats in groups C,D,E,and F were increased(P<0.05).In addition,the levels of oxidative stress,myocardial apoptosis,and PI3K and AKT expression

关 键 词：冠状动脉粥样硬化性心脏病 益心通脉颗粒 应激水平 心肌损伤 

分 类 号：R541.4[医药卫生—心血管疾病]