Agronomic evaluation of Herbaspirillum seropedicae strain ZAE94 as an inoculant to improve maize yield in Brazil  

作　　者：Gabriela C.ALVES Carlos L.R.DOS SANTOS Jerri E.ZILLI Fabio B.DOS REIS JUNIOR Ivanildo E.MARRIEL Farley A.da F.BREDA Robert M.BODDEY Veronica M.REIS 

机构地区：[1]Federal Rural University of Rio de Janeiro,Department of Soils,km 7 BR 465,Seropédica RJ 23900-000(Brazil) [2]Federal University of Mato Grosso,2367 Fernando Corrêa Costa Avenue,Boa Esperança M 78060-900(Brazil) [3]Embrapa Agrobiology,km 07 BR 465,Seropédica RJ 23891-000(Brazil) [4]Embrapa Cerrados,BR-020 km 18,Planaltina DF 73310-970(Brazil) [5]Embrapa Maize and Sorghum,MG 424 km 45,Sete Lagoas MG 35701-970(Brazil)

出　　处：《Pedosphere》2021年第4期583-595,共13页土壤圈（英文版）

基　　金：funded by Brazilian Agriculture Research Corporation—Embrapa,the National Research Council (CNPq),Brazil (No.465133/2014-2);Newton Fund “Understanding and Exploiting Biological Nitrogen Fixation for Improvement of Brazilian Agriculture” (No.B/N012476/1);the Biotechnology and Biological Sciences Research Council (BBSRC),Brazil;the Brazilian National Council for State Funding Agencies (CONFAP);the Coordination of Improvement of Higher Education Personnel (CAPES),Brazil (No.001)。

摘　　要：Diazotrophic bacteria applied as a seed inoculant can improve the grain yield of several crops including maize. The current study aimed to test the agronomic efficiency and contribution of biological nitrogen fixation(BNF) of the endophytic diazotroph Herbaspirillum seropedicae strain ZAE94 to maize under field conditions. Eighteen field assays were conducted in four different locations during consecutive years on two hybrids and two varieties of maize in a random block design with four replicates using a peat-based inoculant. The inoculant containing the ZAE94 strain was applied without nitrogen(N)fertilization or with 40 kg N ha^(-1) and was compared to the application of 40 and 80 kg N ha^(-1) without inoculation. Crop productivity and N accumulation in the grain were evaluated in addition to ^(15)N natural abundance(δ^(15)N) to evaluate BNF in the treatments without N fertilization. Fertilization at 40 kg N ha^(-1) plus bacterial inoculation produced crop yields similar to the treatment with 80 kg N ha^(-1) and increased grain N content, especially in the off-season with 40 kg N ha^(-1). The inoculation treatments showed lower δ^(15)N values than the non-inoculated treatments, which was most evident in the off-season. The BNF contributed about 30% of N accumulated in plants inoculated with ZAE94. On average, 64% of the N fertilized plots showed an increase of the parameters evaluated in the inoculated treatments, compared with the control. Inoculation also increased root length, root volume, and leaf area, and these parameters were positively correlated with plant weight using a hydroponic assay. This study revealed that the application of H. seropedicae inoculant increased the amount of N in plants owing to BNF, and there is a better chance of yield response to inoculation under low N fertilizer application in the off-season.

关 键 词：agronomic efficiency biological nitrogen fixation endophytic diazotroph inoculation 15N natural abundance plant growth-promoting bacteria 

分 类 号：S513[农业科学—作物学]