Three functionally redundant plant-specific paralogs are core subunits of the SAGA histone acetyltransferase complex in Arabidopsis  

作　　者：Chan-Juan Wu Zhen-Zhen Liu Long Wei Jin-Xing Zhou Xue-Wei Cai Yin-Na Su Lin Li She Chen Xin-Jian He 

机构地区：[1]College of Life Sciences,Beijing Normal University,Beijing,China [2]National Institute of Biological Sciences,Beijing 102206,China [3]Tsinghua Institute of Multidisciplinary Biomedical Research,Tsinghua University,Beijing 10084,China

出　　处：《Molecular Plant》2021年第7期1071-1087,共17页分子植物（英文版）

基　　金：the National Natural Science Foundation of China(32025003);the National Key Research and Development Program of China(2016YFA0500801)from the Chinese Ministry of Science and Technology.

摘　　要：The SAGA(Spt-Ada-Gcn5 acetyltransferase)complex is an evolutionarily conserved histone acetyltransferase complex that has a critical role in histone acetylation,gene expression,and various developmental processes in eukaryotes.However,little is known about the composition and function of the SAGA complex in plants.In this study,we found that the SAGA complex in Arabidopsis thaliana contains not only conserved subunits but also four plant-specific subunits:three functionally redundant paralogs,SCSI,SCS2A,and SCS2B(SCS1/2A/2B),and a TAF-like subunit,TAFL.Mutations in SCS1/2A/2B lead to defective phenotypes similar to those caused by mutations in the genes encoding conserved SAGA subunits HAG1 and ADA2B,including delayed juvenile-to-adult phase transition,late flowering,and increased trichome density.Furthermore,we demonstrated that SCS1/2A/2B are required for the function of the SAGA complex in histone acetylation,thereby promoting the transcription of development-related genes.These results together suggest that SCS1/2A/2B are core subunits of the SAGA complex in Arabidopsis.Compared with SAGA complexes in other eukaryotes,the SAGA complexes in plants have evolved unique features that are necessary for normal growth and development.

关 键 词：histone acetylation SAGA development SCSI HAG1 ADA2B 

分 类 号：Q94[生物学—植物学]