四种十字花科植物CONSTANS-like基因家族的鉴定和进化分析  

Identification and evolutionary analysis of CONSTANS-like gene family in four cruciferous plants

作　　者：刘丹青 金玉环 郭力 李永光 黄先忠 LIU Danqing;JIN Yuhuan;GUO Li;LI Yongguang;HUANG Xianzhong(Plant Genomics Lab,Shihezi University,Shihezi,Xinjiang 832003,China;Center for Crop Biotechnology,Agricultural college,Anhui Science and Technology University,Fengyang,Anhui 233100,China;College of Life Sciences,Shihezi University,Shihezi,Xinjiang 832003,China)

机构地区：[1]石河子大学植物基因组学实验室,新疆石河子832003 [2]安徽科技学院农学院,作物生物技术研究中心,安徽凤阳233100 [3]石河子大学生命科学学院,新疆石河子832003

出　　处：《植物生理学报》2021年第6期1241-1260,共20页Plant Physiology Journal

基　　金：国家自然科学基金(U1303302);石河子大学国际合作项目(GJHZ201806);安徽科技学院学科带头人引进人才启动经费(NXYJ202001)。

摘　　要：CONSTANS(CO)和CONSTANS-like(COL)基因介于生物节律钟与下游开花控制基因之间,编码的蛋白质是参与调控植物生长发育及响应逆境胁迫的光周期转录因子。本研究通过全基因组分析从拟南芥(Arabidopsis thaliana)、琴叶拟南芥(Arabidopsis lyrata)、小鼠耳芥(Arabidopsis pumila)和亚麻荠(Camelina sativa)中分别鉴定出17、17、34和51个COL基因。系统进化分为3个亚组,每组COL蛋白质均具有典型的B-box和CCT结构域;COL基因家族的扩张以片段重复或全基因组复制事件为主,且存在串联重复。比较进化分析表明4种植物的COL同源基因受到较强的纯化选择。利用小鼠耳芥RNA-Seq数据和qRT-PCR分析表明ApCOL基因家族成员的表达响应盐胁迫,暗示COL基因参与盐胁迫抗性。节律表达分析显示Ap-COL1.1/1.2、ApCOL2.1/2.2和ApCOL5.1/5.2在短日照条件下表现出明显的昼夜节律表达模式。本研究揭示了十字花科植物COL基因的进化规律,并为深入研究该家族基因在植物胁迫和开花调控中的作用提供线索。CONSTANS(CO)and CONSTANS-like(COL)genes are located between the circadian clock and downstream flowering control genes.CO proteins are photoperiodic transcription factors involved in regulating plant growth and development and responding to stress.A total of 17,17,34,and 51 COL genes were identified from Arabidopsis thaliana,Arabidopsis lyrata,Arabidopsis pumila,and Camelina sativa using a genome-wide analysis method.Phylogenetic analysis showed that these genes were divided into three groups,and all COL proteins contained B-box and CCT domains.The expansion of the COL gene family was dominated by segmental duplication or whole-genome duplication events,and tandem duplications also existed in COL gene family.Comparative evolution analysis showed that the COL homologous genes of the four plant species were subject to strong purification selection.Gene expression analysis using RNA-Seq data and qRT-PCR showed that ApCOL expressions significantly responded to salt stress,suggesting their potential roles in plant salt-tolerance.Moreover,ApCOL1.1/1.2,ApCOL2.1/2.2,and Ap-COL5.1/5.2 transcriptions displayed diurnal oscillations under short-day conditions.This study revealed that the evolutionary laws of COL gene family in cruciferous plants,and provided clues for further study of the roles of COL genes in plant stress and flowering regulation.

关 键 词：CONSTANS 盐胁迫 昼夜节律 基因表达 

分 类 号：Q943.2[生物学—植物学]