类受体蛋白激酶PBL28参与N-癸酰基高丝氨酸内酯对拟南芥根生长的调控  

Involvement of receptor-like protein kinase PBL28 in the regulation of root growth by N-decanoyl-homoserine lactone in Arabidopsis

作　　者：孙雅娜 何子玮 曹向宇[2,3] 赵芊 刘方[2,3] 贾振华 宋水山[2,3] 张利平 SUN Yana;HE Ziwei;CAO Xiangyu;ZHAO Qian;LIU Fang;JIA Zhenhua;SONG Shuishan;ZHANG Liping(College of Life Sciences,Hebei University,Institute of Life Science and Green Development,Baoding,Hebei 071002,China;Biology Institute,Hebei Academy of Sciences,Shijiazhuang 050051,China;Hebei Engineering and Technology Center of Microbiological Control on Main Crop Disease,Shijiazhuang 050051,China)

机构地区：[1]河北大学生命科学学院,生命科学与绿色发展研究院,河北保定071002 [2]河北省科学院生物研究所,石家庄050051 [3]河北省主要农作物微生物病害控制工程技术研究中心,石家庄050051

出　　处：《植物生理学报》2021年第6期1271-1280,共10页Plant Physiology Journal

基　　金：国家重点基础研究计划项目(2015CB150600);国家自然科学基金(31601144)。

摘　　要：N-酰基高丝氨酸内酯(N-acyl-homoserine lactones,AHLs)是一种细菌群体感应信号分子,由其介导的群体感应(quorum sensing,QS)参与革兰氏阴性菌多种生物学功能的调控。N-癸酰基高丝氨酸内酯(N-decanoyl-homoserine lactone,C10-HSL)是一种中长链的AHLs。前期研究表明,C10-HSL处理可以显著改变植物的根系结构,并且NO和H2O2参与C10-HSL影响植物根系结构的过程。但对于植物如何感知C10-HSL知之甚少。植物中存在大量类受体蛋白激酶,在植物感应外界环境信息调节其自身生长发育过程中发挥重要作用。但植物类受体蛋白激酶是否参与植物感应细菌QS信号分子,进而调控植物生理过程尚不清楚。该研究筛选出了对C10-HSL处理抑制根长表型不敏感的拟南芥类受体蛋白激酶缺失突变体pbl28,发现C10-HSL对突变体pbl28主根生长的抑制、侧根形成的促进效应与野生型拟南芥Col-0相比明显减弱。进一步分析表明,突变体pbl28中C10-HSL诱导产生的NO和H2O2的浓度显著低于Col-0。研究结果初步表明,植物类受体蛋白激酶PBL28可能参与植物对C10-HSL的感知,并且通过调控NO和H2O2的产生影响C10-HSL对拟南芥根系结构的调控。N-acyl homoserine lactones(AHLs)is a quorum-sensing signal molecule in bacteria.Quorum sensing(QS)mediated by AHLs participates in the regulation of various biological processes of Gram-neg ative bacteria.N-decanoyl-homoserine lactone(C10-HSL)is one type of AHL with a medium-length chain.Previous studies have shown that C10-HSL treatment can significantly change the root structure of plants while NO and H2O2 participate in the process of C10-HSL affecting plant root structure.However,little is known about how plants perceive C10-HSL.There exist a large number of receptor-like protein kinases in plants,which play an important role in sensing the outside world to facilitate the regulation of plant development.However,it is unclear whether plant receptor-like protein kinases are involved in plant sensing bacterial QS signal molecules and regulating plant physiological processes.The current study isolated a mutant of Arabidopsis receptor-like protein kinase PBL28 showing the rather low sensitivity to C10-HSL with respect to root growth compared to wild-type Arabidopsis Col-0.It was found that the inhibitory effect on primary root growth and the stimulatory effect on formation of lateral roots of C10-HSL in wild type Arabidopsis were significantly alleviated in the mutant pbl28.Furthermore,the data showed that the C10-HSLinduced increase in production of NO and H2O2 in mutant pbl28 was remarkably lower than those in wild type Arabidopsis Col-0.The preliminary results indicate that the plant receptor-like protein kinase PBL28 might be involved in the perception of plant to C10-HSL and participate in the regulation of Arabidopsis root structure by C10-HSL via mediating the production of NO and H2O2.

关 键 词：N-癸酰基高丝氨酸内酯 拟南芥 pbl28 类受体蛋白激酶 

分 类 号：Q945[生物学—植物学]