温郁金脱毒快繁及移栽方法研究  

Study on the Rapid Propagation and Transplanting Methods of Virus-free Seedling of Curcuma wenyujin

作　　者：郭月琴 李帆 姜程曦 任仙樱 GUO Yue-qin;LI Fan;JIANG Cheng-xi(College of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,Zhejiang 325035;Institute of Life Sciences,Wenzhou University,Wenzhou,Zhejiang 325035;Wenzhou Ejin Pharmaceutical Co.,Ltd.,Yueqing,Zhejiang 325600)

机构地区：[1]温州医科大学药学院,浙江温州325035 [2]温州大学生命科学研究院,浙江温州325035 [3]温州莪金医药有限公司,浙江乐清325600

出　　处：《安徽农业科学》2021年第16期40-43,共4页Journal of Anhui Agricultural Sciences

基　　金：合肥市未来药物开发有限公司横向课题(KJHX2008);云南大理药业股份有限公司横向课题(KJHX1603)。

摘　　要：[目的]通过试验寻找出优良的温郁金脱毒苗的制备方法及栽培方法。[方法]通过固定其中一个条件不变,改变其他因素,验证出较好的温郁金根状茎消毒方法,及其丛生芽的诱导、愈伤、继代、分化、生根培养方法。[结果]温郁金根状茎的消毒方法为流水冲洗30~50 min,再用70%乙醇和10%过氧化氢先后消毒20~40 s和6~8 min,然后用无菌水重新冲洗3~4次。丛生芽诱导培养基为MS+6-BA 3.0 mg/L+NAA 1.0 mg/L,愈伤、继代、分化、生根培养基分别为MS+2,4-D 2.0 mg/L+KT 5.0 mg/L、MS+2,4-D 1.0 mg/L+KT 0.5 mg/L、MS+KT 5.0 mg/L+NAA 0.3 mg/L、MS+NAA 1.0 mg/L。[结论]通过对脱毒苗制备过程的条件控制,使温郁金脱毒苗品质较高以及脱毒率可以达到100%,另外提供科学的栽培方法,使所制备的脱毒苗栽培育苗后成活率达到100%,且幼苗健壮。[Objective]The preparation method and cultivation method of excellent Curcuma wenyujin virus-free seedlings were found through experiments.[Method]By fixing one of the conditions unchanged and changing other factors,a better disinfection method of Curcuma wenyujin rhizomes,and the induction,callus,subculture,differentiation,and rooting culture methods of cluster buds were verified.[Result]The disinfection method of Curcuma wenyujin rhizoma tympanum was to flush the rhizoma tympanum by running water for 30-50 minutes,then disinfect it with 70%ethanol and 10%hydrogen peroxide for 20-40 s and 6-8 minutes successively,and then rinse it again with sterile water for 3-4 times.The cluster bud induction medium was MS+6-BA 3.0 mg/L+NAA 1.0 mg/L.The media for callus,subculture,differentiation and rooting were MS+2,4-D 2.0 mg/L+KT 5.0 mg/L,MS+2,4-D 1.0 mg/L+KT 0.5 mg/L,MS+KT 5.0 mg/L+NAA 0.3 mg/L,MS+NAA 1.0 mg/L.[Conclusion]By controlling the conditions in the preparation process of virus-free seedlings,the quality of Curcuma wenyujin virus-free seedlings is higher and the virus-free rate can reach 100%,and by providing scientific cultivation methods,the survival rate of the prepared virus-free seedlings after cultivation and nursery can reach 100%,and the seedlings are robust.

关 键 词：温郁金 脱毒苗 制备方法 移栽方法 

分 类 号：S567.23[农业科学—中草药栽培]