黑皮鸡枞的分子鉴定与营养需求研究  

Study of Molecular Identification and Nutritional Requirement of Hymenopellis raphanipes

作　　者：罗影[1] 关永强 贾培松[1] 赵振豪 努尔孜亚·亚力买买提[1] 魏鹏[1] 贾文捷[1] LUO Ying;GUAN Yong-qiang;JIA Pei-song(Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi,Xinjiang 830091;Xinjiang Institute of Chinese Materia Medica and Ethnodrug,Urumqi,Xinjiang 830002)

机构地区：[1]新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,新疆乌鲁木齐830091 [2]新疆维吾尔自治区中药民族药研究所,新疆乌鲁木齐830002 [3]新疆农业大学,新疆乌鲁木齐830091

出　　处：《安徽农业科学》2021年第16期44-50,共7页Journal of Anhui Agricultural Sciences

基　　金：国家现代农业产业技术体系项目(CARS-20)。

摘　　要：为了明确黑皮鸡枞的营养需求,促进其制种工艺的提高。以新疆麦盖提县人工栽培的菌株为试材,采用单因素试验分别对其母种和原种的营养需求进行试验。结果显示,黑皮鸡枞母种培养基中最佳碳源为麦芽糖,最佳氮源为酵母浸粉,加入1%的MgSO_(4)、CaCl_(2)及枣粉均能促进其菌丝生长,而NaCl和KH 2PO_(4)抑制其菌丝生长,菌丝培养最适温度为20~28℃,最适pH为5.5~6.0;原种培养基中将棉籽壳与木屑以7∶3比例混合作为主料使用效果明显。To identify the effects of different nutrients on the mycelical growth of Hymenopellis raphanipes,and improve the level of strains production.The test was studied by single factor plate experiment,using a Hymenopellis raphanipes strain from Maigaiti County of Xinjiang as material.The results showed that the best carbon and nitrogen source were maltose and yeast extract respectively.The addition of 1%MgSO_(4),CaCl_(2)and jujube powder could promote the mycelial growth,while NaCl and KH 2PO_(4)inhibited it.The optimal temperature range was 20-28℃,and the optimal pH was 5.5-6.0.The mixture of cottonseed hull and sawdust at the ratio of 7∶3 was used as the main material.

关 键 词：黑皮鸡枞 ITS 营养需求 

分 类 号：S646[农业科学—蔬菜学]