内生Bacillus svelezensis HBB5菌株发酵盾叶薯蓣产薯蓣皂苷元的研究  

The production of diosgenin by endophytic Bacillus svelezensis HBB5 through fermenting Dioscorea zingiberensis C.H.Wright

作　　者：王祖华 徐启燃 周顺丽 金志雄 WANG Zu-hua;XU Qi-ran;ZHOU Shun-li;JIN Zhi-xiong(Taihe Hospital,Hubei University of Medicine,Shiyan,Hubei 442000,China;不详)

机构地区：[1]湖北医药学院附属太和医院,湖北十堰442000 [2]湖北医药学院附属东风医院

出　　处：《中国微生态学杂志》2021年第7期774-778,共5页Chinese Journal of Microecology

基　　金：湖北医药学院药护学院创新团队项目(2016YHKT02)。

摘　　要：目的探讨内生Bacillus svelezensis HBB5菌株发酵宿主植物盾叶薯蓣产薯蓣皂苷元的能力。方法接种内生B.svelezensis HBB5及B.subtilis ATCC 6633菌株(0.35×10^(8) CFU/mL)至含盾叶薯蓣地下茎组织的液体培养基,32℃、165~185 r/min连续发酵108 h,检测发酵液细菌、pH、淀粉、麦芽糖、葡萄糖、淀粉酶(α-amylase)及薯蓣皂苷元溶出率等指标。结果内生B.svelezensis HBB5菌株有较强的酸、碱耐受力[pH(4.8±0.2)~(8.4±0.2)],相比B.subtilis ATCC 6633[pH(5.2±0.2)~(8.7±0.2)]差异不明显;前者达峰值生长量(60×10^(8±2) CFU/mL)明显高于后者(32×10^(8±2) CFU/mL)。发酵36~60 h时,B.svelezensis HBB5、B.subtilis ATCC 6633菌株发酵液的淀粉、麦芽糖、葡萄糖浓度达峰值,分别为(37.41±3.12)、(27.83±2.14)ng/mL,(21.06±1.25)、(16.54±1.08)ng/mL,(54.33±3.12)、(36.65±2.10)ng/mL,前者均高于后者。同时,B.svelezensis HBB5菌株维持高的α-amylase酶活性及薯蓣皂苷元溶出率。结论内生B.svelezensis HBB5菌株拥有较强的耐酸碱、降解淀粉、提高薯蓣皂苷元溶出的能力,为工业生产薯蓣皂苷元提供了一个新的方法。Objective To study the production of diosgenin from Dioscorea zingiberensis by endophytic Bacillus svelezensis HBB5 strain. Methods Endophytic B. svelezensis HBB5 and B. subtilis ATCC 6633(0.35×10^(8) CFU/mL) were inoculated into the liquid medium containing dry powder of D. zingiberensis at 32 ℃, 165-185 r/min, and continuously fermented for 108 hours. The indexes of bacteria, pH, starch, maltose, glucose, α-amylase and Diosgenin were detected. Results Endophytic B. svelezensis HBB5 showed strong acid and alkali tolerances [pH(4.8±0.2)-(8.4±0.2)], but there was no significant difference compared with B. subtilis ATCC 6633 [pH(5.2±0.2)-(8.7±0.2)]. The peak growth of the former(60×10^(8±2) CFU/mL) was significantly higher than that of the latter(32×10^(8±2) CFU/mL). The concentrations of starch, maltose and glucose by B. svelezensis HBB5 vs B. subtilis ATCC 6633 were the highest at 36-60 hours, which were(37.41±3.12)ng/mL vs(27.83±2.14)ng/mL,(21.06±1.25)ng/mL vs(16.54±1.08)ng/mL, and(54.33±3.12)ng/mL vs(36.65±2.10)ng/mL, respectively, with the former superior to the latter. Meanwhile, B. svelezensis HBB5 maintained a high α-amylase activity and Diosgenin dissolution rate. Conclusion Endophytic B. svelezensis HBB5 has strong acid and alkali resistance, high starch degradation, and the ability to improve the dissolution of diosgenin, which provides a new method for industrial production of diosgenin.

关 键 词：盾叶薯蓣 内生细菌 薯蓣皂苷元 

分 类 号：Q939.95[生物学—微生物学]